PRODUCTS
CTS™ DynaCellect™ Magnetic Separation System
The Gibco™ CTS™ DynaCellect™ Magnetic Separation System is an automated instrument for T-cell isolation and activation, as well as magnetic bead removal. The instrument utilizes an integrated magnet-rocker and fluidics management for target-cell separation and subsequent bead removal in a closed system. The key design feature is the user-programmable software, which enables design flexibility, performance precision, and scalability from process development to commercial manufacturing. Together with the fit-for-purpose, sterile, single-use consumables, it delivers high cell purity, recovery, and viability.
Advanced Solutions for CAR T and Cell Therapy Manufacturing
The CTS DynaCellect System is specifically engineered to meet the rigorous demands of CAR T cell therapy and other advanced immunotherapies. By providing a closed and automated workflow, it addresses critical challenges in cell therapy manufacturing, ensuring high-quality starting material for gene modification and expansion. Whether you are working on autologous or allogeneic cell-based products, the system provides the robustness needed for reliable therapeutic production.
Isolation Efficiency, Purity, and Viability
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Exceptional Performance: Enables >88% isolation efficiency of target cells with 97% purity when used with CTS™ Detachable Dynabeads™ CD3/CD28.
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Cell Integrity: The magnetic separation process maintains cell viability and phenotype throughout the workflow.
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Precision: Delivers highly reproducible results with a coefficient of variation (CV) of 3%.
Integrated Fluidics and Magnetics Management
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Automated Processing: Features intuitive software to manage all instrument actions, including magnet engagement, pinch valve control, and pump speeds.
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Rocker Mechanism: Integrated rocker with adjustable angles (-30° to 30°) and speeds (1–30 cpm) for optimal cell and bead interaction.
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Flow Control: Precise fluidics management with pump speeds ranging from 1 to 150 mL/min.
Versatile Scalability and Speed
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Scalable Volumes: Supports a flexible reaction volume from 10 mL up to a 1,000 mL maximum working volume, equivalent to approximately 10 billion T cells as input for cell isolation.
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Rapid Isolation: Typical processing time for isolation is between 70 and 100 minutes.
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High-Volume Bead Removal: Verified bead removal for volumes up to 10 L using a continuous flow process.
GMP-Compliant Manufacturing and Connectivity
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Sterile Environment: Closed, single-use kits enable processing within Class C cleanroom facilities.
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Regulatory Compliance: Manufactured under ISO 13485 standards with an optional software module for 21 CFR Part 11 compliance.
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Digital Integration: Features an OPC-UA interface for seamless communication with DeltaV™ systems and MES/LIMS platforms.
CTS™ Rotea™ Counterflow Centrifugation System
The Gibco™ CTS™ Rotea™ Counterflow Centrifugation System is an award-winning, highly versatile tool designed for cell separation, concentration, washing, and buffer exchange. Utilizing innovative Counterflow Centrifugation (CFC) technology, the system suspends cells in a fluidized bed by exerting a constant flow force opposing centrifugal forces. This unique mechanism provides capabilities beyond traditional centrifuges, delivering high cell recovery and viability in a closed, automated environment from process development through commercialization.
Versatile Applications in Cell and Gene Therapy
The CTS Rotea System is engineered to support a broad range of functionalities across the cell therapy workflow:
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Advanced Cell Processing: Optimized for T cell, NK cell, and MSC wash and concentration.
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Specialized Harvesting: Enables MSC harvesting from Cell Factory Systems and iPSC aggregate processing.
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Viral Vector Manufacturing: Suitable for Lentiviral vector clarification and residual wash out applications.
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Precision Separations: Facilitates PBMC/monocyte separation, RBC depletion, and elutriation.
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Quality & Research: Ideal for QC sample prep, isolation, and small- to mid-scale cell processing.
High Efficiency and Integrated Technology
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Superior Recovery and Viability: Gentle processing enables >95% cell recovery while maintaining high cell viability.
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Low-Volume Output: Proprietary DirectDraw extraction technology can deliver as little as 5 mL of concentrate, providing highly concentrated cell populations.
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Live Visualization: Integrated Gibco™ CellCam™ video technology allows users to visualize cells live in the fluidized bed for precise parameter optimization.
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Customer-Controlled Development: User-friendly software enables the creation, modification, and assessment of custom protocols to suit unique applications.
Scalability and Speed
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Flexible Input Range: Supports a wide recommended input volume range from 50 mL up to 20 L.
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Rapid Processing: Capable of washing and concentrating 1 L of cells in approximately 18 minutes or isolating PBMCs from a full leukopak bag in about 85 minutes.
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Modular Integration: Designed for easy integration into existing workflows, allowing for a seamless transition from research to GMP manufacturing.
GMP-Compliant Manufacturing and Connectivity
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Sterile, Closed System: Single-use kits enable sterile processing in Class C cleanroom facilities, reducing the requirement for high-grade lab space by up to 90%.
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Regulatory Support: Manufactured in an ISO 13485-certified facility with software options designed to help enable 21 CFR Part 11 compliance.
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Digital Connectivity: Features an OPC-UA interface for connection to MES/LIMS platforms and available CTS™ Cellmation™ Software for integration with DeltaV™ systems.
CTS™ Compleo™ Fill and Finish System
Automated, flexible formulation and fill system designed for cell therapy manufacturing
The Gibco™ CTS™ Compleo™ Fill and Finish System is a functionally closed, automated system that reduces variability to help better control formulation and filling as compared to manual processing. It enables solutions to help overcome common challenges that arise during the manual workflow, streamlining the process from process development to commercial manufacturing.
Key Features and Benefits
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Functionally Closed Automation: Reduces formulation and fill process variability while maintaining aseptic integrity through closed-system processing.
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Intelligent Formulation Software: A proprietary algorithm automates complex calculations, reducing manual errors and optimizing cell usage to minimize unnecessary cell waste.
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High Dosing Accuracy: Delivers a volumetric accuracy of ±10% even at ultra-low fill volumes as small as 0.5 mL.
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Modular Integration: Designed to work in harmony with the CTS™ Rotea™ system, allowing the output bag to be placed directly onto the Compleo™ system.
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System-Agnostic Flexibility: Can be implemented with other cell processing platforms to enhance formulation and filling efficiency across diverse workflows.
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Operational Speed: Automates air removal and processing, reducing formulation and filling time from an average of 45 minutes to approximately 15 minutes.
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GMP-Ready Compliance: Supports 21 CFR Part 11 compliance via an optional SAE software solution and an OPC-UA interface for seamless integration with DCS/MES systems.
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Real-Time Flexibility: The user-friendly Protocol Builder software handles variable batch sizes in real time without requiring manual protocol modification or requalification.
Technical Specifications
Input Capacity: Up to 4 inputs depending on in-process sample allocation Input Volume: 10 mL (minimum), 750 mL (maximum) Flow Rate: 5–50 mL/min Number of Outputs: 1–10 depending on number of input bags and orientation Output Volume Range: 0.5 mL to 750 mL per individual dose Output Volume Accuracy: ±10% across the full range (0.5 mL to 750 mL) Volume Verification: Independent volume checking mechanism; bubble sensors measure the volume transferred based on the volume of fluid pumped Output Container Compatibility: Compatible with most cryobags and vials; supports connection via luer lock fittings or direct sterile weld to the bag inlet line In-process Sampling: Can dispense multiple samples with as little as 0.2 mL per sample Air Removal (Burping): Automatic (pre- and/or post-fill) and manual Connectivity: OPC-UA interface Operating Noise Level: 65 dBA (typical) to 70 dBA (maximum) Dimensions (H x W x D): 31.5 x 11.4 x 18.0 in (80 x 29 x 46 cm) Weight: 55.0 lb (25.0 kg)
Cell Therapy Solutions
Discover Thermo Fisher Scientific integrated cell therapy solutions to support your development efforts at every stage. Through high-quality tools and services, our goal is to enable you from initial discovery through clinical trials to commercially viable cell therapies.
Cell Therapy Systems (CTS): from high-quality cGMP manufactured cell therapy ancillary materials to regulatory documentation and safety testing, find out how our CTS products can connect your clinical stem cell therapy and immunotherapy research to the clinic, and beyond.
• CTS Media and Supplement GMP manufactured designed for cell and gene therapy to support your transition from discovery through clinical and commercial manufacturing.
• Cell Therapy Instruments, supporting consumables and software products enable CGMP compliant, closed system manufacturing and allow for physical and digital integration.
• Viral vector systems cost-effective, scalable solutions designed to transition your gene therapy workflow from discovery to commercial manufacturing.
Download Cell Therapy Brochure and connect with the solutions and guidance you need to advance your cell therapies.
Transient Protein Expression Platforms
The Expi293 and ExpiCHO transient expression systems offer all-in-one solutions for generating high-titer recombinant proteins for a broad range of research applications including candidate drug identification, reagent production, structural biology and vaccine research and membrane protein biology.
At the research scale, transient protein expression platforms offer protein production labs many advantages over a stable system. Benefits of rapid, high-yield transient protein production, in addition to the obvious yield benefits offered by Expi platforms, labs can help minimize the burden on their staff by reducing hands-on-time needed for protein production, freeing up researchers to perform more experiments and analysis.
Click “Learn more” to select the right system to meet your research and development needs according to host type or application.
Cell Analysis Solutions
Cells constitute discrete units of biological function and serve as starting points in a myriad of studies to identify and map many of the basic biochemical and physical processes of life. Compiling data sets from multiple cell analysis investigations allows scientists to better understand, predict, and—ultimately—influence the factors that underlie cell health, proliferation, function, and death.
We offer a broad range of cell analysis research solutions including Gibco cell culture media and reagents, Invitrogen labeling and detection technologies, antibodies and immunoassays, Invitrogen EVOS cell imaging systems, Invitrogen Countess 3/3FL Automated Cell Counters, and the Invitrogen Attune acoustic focusing flow cytometers.
Featured cell analysis categories:
Cell Imaging – reagents and kits for cellular imaging applications such as fluorescence microscopy, IHC, ISH, HCS, LCM, and small animal in vivo imaging.
Cell Function Assays – Functional assays and antibodies for the analysis of cell health—from apoptosis to autophagy, cell cycle, viability, proliferation, angiogenesis, and more.
Flow Cytometry – Antibodies, cell functional reagents, Invitrogen Attune flow cytometers, and instrument calibration products.
Click “Learn more” to browse our broad cell analysis portfolio and interactive selection tools.
Sample preparation – Nucleic Acid Isolation
Nucleic acid isolation is a crucial first step in the molecular biology workflow, whether you are isolating genomic DNA (gDNA) or RNA. Selecting nucleic acid purification products that are optimized to provide maximum yield, purity, and integrity from virtually any sample type and application is important for your research success.
We offer complete solution portfolio for sample extraction and preparation:
• Magnetic beads: 0.5–1.0 μm particles with a paramagnetic core and modified shell (e.g., Applied Biosystems™ MagMAX™ kits and Invitrogen™ Dynabeads™ magnetic beads) Samples are lysed in solution and allowed to bind nucleic acid to magnetic particles based on specific surface modifications. Application of an external magnetic field rapidly collects the particles. Rounds of release, wash, and recapture enable purification of the desired nucleic acid.
• Spin columns: Glass fiber, derivatized silica, or ion exchange membrane in column (e.g., Thermo Scientific™ GeneJET™ and Invitrogen™ PureLink™ kits) Samples are lysed and passed through the membrane using centrifugal or vacuum force. Wash and elution solutions are subsequently passed through the membrane, and the sample is collected into a tube by centrifugation.
• Organic extraction: Phenol-chloroform solution (e.g., Invitrogen™ DNAzol™ and TRIzol™ Reagents) After homogenizing the sample with TRIzol Reagent, chloroform is added, and the mixture separates into a clear upper aqueous layer containing RNA, an interphase layer, and a pink lower organic layer containing the DNA and protein. RNA is precipitated from the upper aqueous layer with isopropanol. DNA is precipitated from the interphase and organic layers with ethanol. Protein is precipitated from the phenol–ethanol supernatant with isopropanol.
KingFisher Automation
KingFisher purification instruments automate the extraction and purification of DNA, RNA, proteins, and cells. KingFisher purification instrument models serve applications ranging from SARS-CoV-2 and infectious diseases, extracting viral or pathogen nucleic acid, to isolating genomic DNA (gDNA) for genetic testing. Their broad functionality and customizable software feature additionally serve applications, such as FFPE and cell-free DNA (cfDNA) tissue and liquid biopsy, immunoprecipitation, peptide mapping, and cells and exosomes.
Reverse Transcription
Reverse transcription is the synthesis of complementary DNA (cDNA) from an RNA template using reverse transcriptase enzymes. The cDNA can be used as a template for PCR amplification, cDNA library construction, RNA sequencing, and more. Selecting the right reverse transcriptase is critical to detecting low-abundance RNAs in a sample and obtaining high yields of full-length cDNA.
Choose from a variety of reverse transcriptase stand-alone enzymes, master mixes, cDNA synthesis kits, primers, and dNTP solutions to suit your application. Invitrogen SuperScript IV products aid in exceptional cDNA synthesis from any type of template RNA.
Click “Learn more” for reverse transcription reagent selection guide.
PCR Reagents and Enzymes
Choose from a variety of PCR enzymes and reagents for your applications, with the flexibility needed to perform your experiments.
With PCR enzymes you know and trust, such as, Applied Biosystems AmpliTaq and AmpliTaq Gold, Invitrogen Platinum II Taq , and Platinum SuperFi II DNA polymerases, we have what it takes for successful PCR.
Click “Learn more” for interactive PCR Enzymes Selection Tool.
Molecular Cloning Essentials
Innovative products for the molecular cloning workflow:
The molecular cloning workflow requires multiple steps to obtain high-quality cloned DNA for your downstream applications. Help advance your molecular cloning workflow at every step and accelerate construction of recombinant clones. Our wide range of cloning solutions address needs from routine to specialty applications, helping you find optimal reagents to achieve fast results with greater confidence.
Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery, among the reputable solution: GeneArt Gibson Assembly Cloning; Gateway Cloning Technology; TOPO PCR Cloning and more.
Click on each step to explore the molecular cloning workflow and select products for your downstream applications that fit your research.
MiniAmp Thermal Cyclers: Compact Size, Elevated Routine PCR
Our thermocyclers are designed for reliability, accuracy, and user friendliness to fit scientists’ needs. Applied Biosystems PCR instruments are equipped with:
• VeriFlex temperature control technology with segmented blocks that allow for precise temperature control.
• Thermal Cycler Fleet Control Software—manage multiple instruments with the help of optional software to help enhance productivity of PCR machines with this secure remote access feature.
Backed by the proven reliability of Applied Biosystems technology the MiniAmp Plus Thermal Cycler and the MiniAmp Thermal Cycler were designed for routine PCR. With their compact design, just 19 cm wide, and entry-level price, MiniAmp Thermal Cyclers are perfect for every lab bench.
The MiniAmp Thermal Cycler has an isothermal block, while the MiniAmp Plus Thermal Cycler offers an upgrade to a VeriFlex Block for PCR optimization.
