CTS™ Rotea™ Counterflow Centrifugation System

The Gibco™ CTS™ Rotea™ Counterflow Centrifugation System is an award-winning, highly versatile tool designed for cell separation, concentration, washing, and buffer exchange. Utilizing innovative Counterflow Centrifugation (CFC) technology, the system suspends cells in a fluidized bed by exerting a constant flow force opposing centrifugal forces. This unique mechanism provides capabilities beyond traditional centrifuges, delivering high cell recovery and viability in a closed, automated environment from process development through commercialization.

Versatile Applications in Cell and Gene Therapy

The CTS Rotea System is engineered to support a broad range of functionalities across the cell therapy workflow:

• Advanced Cell Processing: Optimized for T cell, NK cell, and MSC wash and concentration.

• Specialized Harvesting: Enables MSC harvesting from Cell Factory Systems and iPSC aggregate processing.

• Viral Vector Manufacturing: Suitable for Lentiviral vector clarification and residual wash out applications.

• Precision Separations: Facilitates PBMC/monocyte separation, RBC depletion, and elutriation.

• Quality & Research: Ideal for QC sample prep, isolation, and small- to mid-scale cell processing.

High Efficiency and Integrated Technology

• Superior Recovery and Viability: Gentle processing enables >95% cell recovery while maintaining high cell viability.

• Low-Volume Output: Proprietary DirectDraw extraction technology can deliver as little as 5 mL of concentrate, providing highly concentrated cell populations.

• Live Visualization: Integrated Gibco™ CellCam™ video technology allows users to visualize cells live in the fluidized bed for precise parameter optimization.

• Customer-Controlled Development: User-friendly software enables the creation, modification, and assessment of custom protocols to suit unique applications.

  Scalability and Speed

• Flexible Input Range: Supports a wide recommended input volume range from 50 mL up to 20 L.

• Rapid Processing: Capable of washing and concentrating 1 L of cells in approximately 18 minutes or isolating PBMCs from a full leukopak bag in about 85 minutes.

• Modular Integration: Designed for easy integration into existing workflows, allowing for a seamless transition from research to GMP manufacturing.

GMP-Compliant Manufacturing and Connectivity

• Sterile, Closed System: Single-use kits enable sterile processing in Class C cleanroom facilities, reducing the requirement for high-grade lab space by up to 90%.

• Regulatory Support: Manufactured in an ISO 13485-certified facility with software options designed to help enable 21 CFR Part 11 compliance.

• Digital Connectivity: Features an OPC-UA interface for connection to MES/LIMS platforms and available CTS™ Cellmation™ Software for integration with DeltaV™ systems.