PRODUCTS
Western Blot Detection Reagents
With a variety of detection techniques for western blotting-chemiluminescence, fluorescence, or colorimetric, to choose from, you can select a technology to match your experimental requirements by clicking “Learn more” below.
Chemiluminescent substrates are popular because they offer several advantages over other detection methods. These advantages have allowed chemiluminescence to become the detection method of choice in most protein laboratories. Key highlights: Greatest sensitivity; Detection and quantitation for a large range of protein concentrations.
Fluorescent reagents are growing in popularity for western blotting because they offer increased time savings over chemiluminescent detection and reduced chemical waste compared to both chemiluminescent or chromogenic detection systems. Key highlights: Multiplexing capability; Direct signal.
Luminex Instruments
The Luminex family of systems encompasses four instrument models, each offering distinct multiplexing capabilities, throughput, and read times. These Luminex instruments operate on the quantitative fluorescent flow cytometry principle, leveraging the xMAP magnetic bead technology to detect small quantities of analytes. Used in combination with our ProcartaPlex multiplex assays for protein quantitation or QuantiGene Plex assays for gene expression analysis, the Luminex platform delivers a rapid and cost-effective immunoassay profiling tool. This synergy between Luminex instruments and our advanced multiplex assays enhances efficiency and precision in the detection and quantification of biomolecules.
ProcartaPlex Immunoassays
Invitrogen ProcartaPlex assays are antibody and magnetic bead-based multiplex immunoassays that leverage Luminex xMAP technology to enable the simultaneous detection and quantitation of up to 80 protein targets in a single well with only 6.3–50 µL sample of plasma, serum, cell culture supernatants, or other bodily fluids. The assays cover more than 600 cytokines, chemokines, growth factors, and other protein targets from a range of species.
With a broad menu of preconfigured panels (2- to 80-plex) and customizable options, ProcartaPlex assays provide high flexibility to serve your individual research needs. Whether investigating inflammation, immunology, cytokine profiling, oncology, or infectious diseases, you can rely on the accuracy, sensitivity, reproducibility, and versatility of ProcartaPlex multiplex assays to gain deeper insights and uncover complex biological processes.
Countess 3 and Countess 3 FL instruments
Thermo Fisher Scientific offers two high-performance, automated cell counters designed to meet the needs of any lab for accurate and precise cell counting without the drudgery, wasted time, subjectivity, and variability of manual hemocytometer counting. Developed by expert cell biologists with the aid of artificial intelligence, the Invitrogen Countess 3 and Countess 3 FL instruments are almost a paradox: automated cell counters that think like a cell biologist.
Countess 3 Automated Cell Counters provide highly accurate and precise brightfield and fluorescence cell counts even for challenging cell sample types. Automated features like rapid capture, autosave, and built-in calculators add convenience and time savings. Whether you’ve been counting cells manually or using an early-generation automated counter, the Countess 3 cell counters represent a significant upgrade in counting accuracy and workflow efficiency.
Gibco Cell Culture Media
Cell culture medium is vital to your culture environment as it provides components (e.g., nutrients, growth factors) necessary to support cell growth and function. In addition, media can be formulated to regulate environmental conditions in cell culture, including osmotic pressure and pH. A variety of cell culture media formulations have been developed to support a wide range of cell types and experimental applications.
Time-tested and trusted, our Gibco cell culture media line includes products designed to support the growth and maintenance of a variety of mammalian cells and cell lines. We offer ready-to-use cell culture media as well as powdered and concentrated liquid formulations to fit your experimental setup and budget. For your scale-up needs, Gibco Cell Culture for Bioprocessing provides end-to-end products, process development and customization services to help maximize productivity while reducing costs and time to market.
Click “Learn more” for interactive selection tool to choose from all options including classical and innovative media formulations.
Cell culture reagents and supplements
Designed to deliver reproducibility, Gibco cell culture reagents and suppliments are infused with quality, customer-focused innovation, and service excellence from beginning to end. From the most basic formulations to the newest innovations, Gibco products offer exceptional quality, consistency, and performance—for results that you can count on every day.
Cell Culture Reagents and supplements portfolio include GIBCO’s Antibiotics, Essential Amino Acids and GlutaMAX, Balanced Salt Solutions, Cell Freezing Media, TrypLE Enzymes, Cell Dissociation and Trypsin for Cell Culture, Growth Factors & Cytokines, Water For Injection and more.
Click “Learn more” to browse our broad portfolio essential culture products.
Fetal bovine serum (FBS)
Fetal bovine serum (FBS) offers essential nutrients and growth factors for the maintenance and growth of cultured cells. Cell-based applications are an integral part of basic research, biochemical studies, vaccine development, and drug discovery to name a few. FBS is the most used serum in cell-based applications as it provides the most robust culture system for the widest range of cell types.
Since 1962, Gibco FBS products have been a global market leader. Through Thermo Fisher Scientific’s unwavering commitment to quality, scientists using Gibco FBS have been provided with consistent reliability, service, value, and innovation.
Click “Learn more” and discover the performance differences that Gibco Fetal Bovine Serum can deliver for your research and production. You can also contact us and request a sample.
Transfection Reagents
We offer transfection reagents for DNA, siRNA, RNA, and protein delivery, providing a range of options to suit your transfection experiment:
• DNA delivery—Transient and stable transfection of DNA plasmids. DNA transfection is used to study gene function and regulation, mutational analysis and biochemical characterization of gene products, effects of gene expression on the health and life cycle of cells, as well as for large scale production of proteins for purification and downstream applications.
• RNA delivery—Transient transfection of mRNA or RNAi molecules. mRNA transfection, which allows the production of recombinant proteins without the need for nuclear entry, is useful for short-term studies of gene expression and has emerged as a promising application for vaccine studies. RNAi molecules facilitate gene knockdown, which can be used in analyses of protein function and phenotype, function recovery, pathway analysis, in vivo knockdown, and drug target discovery.
• Protein delivery—CRISPR-Cas9 transfection allows for genome editing in broad applications such as stem cell engineering, gene therapy, tissue and animal disease models, and engineering disease-resistant transgenic plants.
Invitrogen Lipofectamine reagents have become the most referenced transfection reagents with over 50,000 citations. You continue to choose these reagents due to their wide acceptance as the best and most reliable available. Lipofectamine reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use.
Click “Learn more” for interactive transfection selection tool to find products, citations, and protocols optimized for your transfection experiments. Input information on your experiment type, cell line, and payload to unlock solutions.
Neon NxT Electroporation System
The combination of the Invitrogen Neon NxT Electroporation System’s proprietary pipette tip technology, simplified workflow, and flexibility helps reduce sample loss, lowers contamination risk, saves you steps, and can put you in control of your experiments.
Benefits of the Neon NxT Electroporation System:
• Proven transfection efficiency and cell viability—transfect challenging cell lines with high efficiency and high cell viability
• Flexibility when you need it—precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols.
• Enhanced genome editing efficiency—optimized buffer for improved delivery of CRISPR molecules
• Save valuable research time—transfect cells quickly with an easy three step workflow, a single buffer kit and intuitive user interface
• Preserve your precious samples—minimize sample transfer loss and contamination risk
Please contact us for a Demo.
Stem Cell Research
Our complete portfolio of stem cell research products and services allows you to simplify your workflow and enables the continuity, efficiency, and precision that drive discovery.
Featured stem cell categories:
Stem cell culture: expand and maintain stem cells in Gibco serum-free, feeder-free, or xeno-free cell culture systems.
Stem cell engineering: manipulate your cells for downstream applications with Invitrogen reprogramming and gene editing tools.
Stem Cell Characterization: analyze stem cells and their derivatives using a wide range of cellular and molecular characterization methods.
Stem Cell Differentiation: differentiate to your desired cell lineage with easy-to-use, flexible kits, growth factors, and supplements.
3D & Cell Modeling using Stem Cells: access resources and solutions to enable the development of more physiologically relevant and reproducible models for research including 3D cell modeling and disease modeling.
Neurobiology Research
Thermo Fisher reagents and technologies embody a broad technology platform for neuroscience research. Thermo fisher Scientific products encompass all major neural cell types and support a multitude of applications from basic research to therapeutic discovery. Streamline your neurobiology experiments and obtain high quality results with our trusted reagents and technologies, such as B27, Neurobasal media.
Find Gibco Neurobiology compete work flow solutions. Download a digital copy of more than 20 neurobiology validated protocols for neural stem cell culture and differentiation, cell analysis, molecular characterization, and transfection. Review experimental best practices, and troubleshooting advice designed to get you started with your neurobiology research.
CTS™ Xenon™ Electroporation System
The Gibco™ CTS™ Xenon™ Electroporation System is a closed, modular, large-scale platform that offers full control over electroporation parameters for optimal performance. The system delivers exceptional transfection performance and viability, enabling electroporation of up to 2.5 billion cells in a 25 mL run to support both process development and manufacturing work in clinical cell therapy. Utilizing a user-programmable interface, it provides the flexibility needed to optimize protocols for a variety of cell types and payloads, ensuring high-quality results from research through commercialization.
High-Performance, Scalable Non-Viral Delivery for Clinical Manufacturing
The CTS Xenon System is specifically engineered to meet the rigorous demands of CAR T cell therapy and other advanced immunotherapies. By providing a closed and automated non-viral delivery workflow, it addresses critical challenges in cell therapy manufacturing, ensuring high-quality gene modification for both autologous and allogeneic cell-based products. The system allows for efficient delivery of various payloads, including DNA, RNA, and proteins, while providing the robustness needed for reliable therapeutic production.
Exceptional Transfection Performance and Viability
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High Efficiency: Achieve up to 90% gene knockout and up to 80% cell viability when used with Gibco CTS TrueCut Cas9 Protein.
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Proven Performance: Benchmarked against the Invitrogen Neon Transfection System, the CTS Xenon system enables seamless translation to large-scale processing without a drop in performance.
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Rapid Processing: Capable of transfecting up to 2.5 billion T cells in a 25 mL volume in less than 25 minutes.
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Precision Control: Delivers consistent results through an embedded graphical user interface (GUI) that allows for full optimization of electroporation protocols.
Integrated Fluidics and Parameter Management
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Automated Processing: Features intuitive software to manage all instrument actions, including voltage, pulse width, and pulse number.
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Full Control: Users can customize parameters such as voltage (500–2,500 V), pulse width (1–30 ms), and number of pulses (1–10) to suit specific cell types.
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Precooling Technology: Integrated Peltier technology for precooling (10–30°C) ensures optimal temperature control during the electroporation process.
Versatile Scalability and Optimized Consumables
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Scalable Consumables: Offers the CTS Xenon SingleShot chamber for rapid 1 mL process development and the CTS Xenon MultiShot cartridge for consistent, closed-system manufacturing volumes up to 25 mL.
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Flexible Buffers: Compatible with specialized buffers, including CTS Xenon Genome Editing Buffer and Lower Conductivity Buffer, designed specifically for high-energy electroporation needs.
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Seamless Translation: Facilitates a direct path from discovery to clinical manufacturing, allowing for process refinement at small scale before transitioning to large-scale production.
GMP-Compliant Manufacturing and Connectivity
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Sterile Environment: Closed, single-use MultiShot cartridges enable sterile processing within Class C cleanroom facilities.
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Regulatory Compliance: Manufactured in ISO 13485-certified facilities with software options designed to help enable 21 CFR Part 11 compliance.
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Digital Integration: Features an OPC UA interface for seamless communication with MES/LIMS platforms and DeltaV systems via CTS Cellmation Software.
