Sample preparation – Nucleic Acid Isolation
Nucleic acid isolation is a crucial first step in the molecular biology workflow, whether you are isolating genomic DNA (gDNA) or RNA. Selecting nucleic acid purification products that are optimized to provide maximum yield, purity, and integrity from virtually any sample type and application is important for your research success.
We offer complete solution portfolio for sample extraction and preparation:
• Magnetic beads: 0.5–1.0 μm particles with a paramagnetic core and modified shell (e.g., Applied Biosystems™ MagMAX™ kits and Invitrogen™ Dynabeads™ magnetic beads) Samples are lysed in solution and allowed to bind nucleic acid to magnetic particles based on specific surface modifications. Application of an external magnetic field rapidly collects the particles. Rounds of release, wash, and recapture enable purification of the desired nucleic acid.
• Spin columns: Glass fiber, derivatized silica, or ion exchange membrane in column (e.g., Thermo Scientific™ GeneJET™ and Invitrogen™ PureLink™ kits) Samples are lysed and passed through the membrane using centrifugal or vacuum force. Wash and elution solutions are subsequently passed through the membrane, and the sample is collected into a tube by centrifugation.
• Organic extraction: Phenol-chloroform solution (e.g., Invitrogen™ DNAzol™ and TRIzol™ Reagents) After homogenizing the sample with TRIzol Reagent, chloroform is added, and the mixture separates into a clear upper aqueous layer containing RNA, an interphase layer, and a pink lower organic layer containing the DNA and protein. RNA is precipitated from the upper aqueous layer with isopropanol. DNA is precipitated from the interphase and organic layers with ethanol. Protein is precipitated from the phenol–ethanol supernatant with isopropanol.
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