Reverse transcription is the synthesis of complementary DNA (cDNA) from an RNA template using reverse transcriptase enzymes. The cDNA can be used as a template for PCR amplification, cDNA library construction, RNA sequencing, and more. Selecting the right reverse transcriptase is critical to detecting low-abundance RNAs in a sample and obtaining high yields of full-length cDNA.
Choose from a variety of reverse transcriptase stand-alone enzymes, master mixes, cDNA synthesis kits, primers, and dNTP solutions to suit your application. Invitrogen SuperScript IV products aid in exceptional cDNA synthesis from any type of template RNA.
Click “Learn more” for reverse transcription reagent selection guide.
Use the selection tool to find the right products to meet your cDNA synthesis needs.
Invitrogen SuperScript product family is ideal for all cDNA synthesis experiments. SuperScript IV RT offers top-notch performance marked by thermostability, high processivity, and exceptional cDNA yields, and is available in a selection of formats.
First strand cDNA synthesis master mix crafted with VILO (Variable Input, Linear Output) technology is an ideal choice for two-step RT-qPCR applications. This master mix is known for its remarkable sensitivity, exceptional linearity, and short reaction times.
One-step RT-PCR kits are optimized for cDNA synthesis and amplification in a single step. This approach is often preferred for a faster and simpler workflow and is suitable for high throughput applications.
Skip RNA isolation and expedite cDNA synthesis. Direct reverse transcription products allow cell lysis and cDNA synthesis to be performed in a single tube, using as few as single cell samples.
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