Knowledge Center

Application Note

Microdissection to Microarrays: A Complete Solution for Gene Expression Profiling of Precious Tissue Samples

Posted by Isabelle

August 21, 2024

Transcriptional profiling of tissue biopsies using microarrays has become a powerful tool for studying biological mechanisms and their alterations lea...

Tutorial

Real-Time PCR Primer Design

Posted by Isabelle

July 25, 2024

Guidelines and ConsiderationsIf you choose to design your own real-time PCR primers, take into consideration that the efficiency (E) of a PCR reaction...

Tutorial

Essentials of Real Time PCR

Posted by Isabelle

July 29, 2024

Guidelines and ConsiderationsReal-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it oc...

Tutorial

How to Avoid Contamination in Your qPCR Experiments

Posted by Isabelle

July 28, 2024

Great Tips for how to make the best out of your experiments  qPCR, with its high sensitivity, allows researchers to produce millions of copies of a sp...

Article

Unveiling Breakthroughs in Disease Research with Thermo Fisher Scientific’s TaqMan Assay Search by Disease

Posted by Isabelle

July 25, 2024

We are excited to bring to your attention the latest advancements in disease research facilitated by Thermo Fisher Scientific's cutting-edge TaqMan As...

Article

Real-Time PCR or Sanger sequencing?

Posted by Isabelle

July 28, 2024

Two technologies widely used in genomics, both providing very useful information. However, which tool do you need for your project? While RT-PCR or qP...

Application Note

Phagocytosis of Bacteria in a Whole Blood Assay: The Influence of Opsonization on Monocyte and Granulocyte Phagocytosis

Posted by Isabelle

July 9, 2024

Objectives• Discover how the various cells of the adaptive immune system respond to opsonization• Learn to evaluate phagocytosis in single cells using...

Application Note

Rapid Screening Method of Calcium Flux by Flow Cytometry

Posted by Isabelle

July 9, 2024

The unique peristaltic sample delivery system of the CytoFLEX analyzer enables optimized measurements of transient changes in intracellular calcium in...

Application Note

Efficient CAR-T Cell Characterization using Dry DURACloneAntibody Panels and Cytobank Analysis Software

Posted by Isabelle

July 9, 2024

CAR-T cell therapies are becoming a more common approach for treating hematological disorders. Flow cytometry is increasingly being used to comprehens...

Application Note

Analysis of Plant Genome Sizes Using Flow Cytometry

Posted by Isabelle

July 9, 2024

A case study demonstrating dynamic range and measurement linearity Plant Flow CytometryFlow cytometers were originally designed to operate with suspen...

Application Note

How to Use Violet Side Scatter to Detect Nanoparticles on the CytoFLEX Flow Cytometer

Posted by Isabelle

July 9, 2024

The detection of sub-micron particles by flow cytometry becomes increasingly difficult as the particle sizes progress smaller than the wavelength of t...

Application Note

A new approach to nanoscale flow cytometry with theCytoFLEX nano analyzer

Posted by Isabelle

July 9, 2024

Nanoscale flow cytometry is a cutting-edge technique that combines the principles of flow cytometry with nanotechnology. It enables the analysis of pa...

Tutorial

Switching Between Normal Phase To Reversed Phase Protocols

Posted by Isabelle

July 9, 2024

Normal phase solvents order of polarity:hexane < dichloromethane < chloroform < isopropanol < ethanol, etc.Reversed phase solvents order o...

Tutorial

Handling and working with Analytical Standards

Posted by Isabelle

July 9, 2024

The purpose of this Standard Operating Procedure is to establish a standardized procedure of using United States Pharmacopoeia (USP) standards, detail...

Tutorial

Optimizing HPLC Device Performance: Clear Instructions for Proper Use and Maintenance

Posted by Isabelle

July 9, 2024

Before operating an HPLC device, it’s crucial to grasp the fundamental principles of liquid chromatography. This sophisticated instrument operates wit...