PRODUCTS
Extracellular Matrices
To keep cells in a more native state in vitro, it helps to tailor their environment by including extracellular matrix (ECM) components that emulate what the cells experience in vivo. By including a relevant cell culture matrix, you can better recapitulate the in vivo environment in your cell culture model. Gibco extracellular matrices, scaffolds, and proteins provide in vivo-like morphology and physiologically relevant cellular environments for more realistic cell culture models.
- Geltrex products are reduced growth factor basement membrane extracts derived from mouse Engelbreth-Holm-Swarm (EHS) tumors.
- Laminin is the major glycoprotein in basement membranes. Mouse laminin facilitates the attachment and expansion of ESCs, iPSCs, and NSCs.
- Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). This cell culture matrix was designed for use with Essential 8 Medium and has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines.
- Collagen is the most widely used extracellular matrix protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.
- Poly-D-lysine (PDL) is a synthetic ECM widely used to promote cell adhesion of a variety of cell types. PDL is also ideal for finicky cells, such as primary and PSC-derived neurons.
Below you will find overview of the cell lines, culture conditions, and analysis techniques being used in the transition from 2D to 3D cell models.
EVOS M3000 Imaging System
Compact, connected, and easily upgradable
The EVOS M3000 Imaging System is a fully integrated digital inverted benchtop microscope for two-color fluorescence, transmitted light, phase contrast, and color imaging. It is excellent for routine cell/tissue culture imaging applications. This system combines an easy user interface with powerful machine learning capabilities that allow users to quickly verify cell appearance, quality, and growth in seconds.
The EVOS M3000 offers you these important advantages:
- Easy installation—no maintenance, assembly, alignment, or calibration
- Automated real-time confluency analysis—includes pre-trained machine learning algorithm that can report cell confluency in the field of view at any time. As vessel is moved, confluency value automatically updates (in under a second on average).
- User-defined two-color fluorescence—with addition of EVOS light cubes, two fluorescence channels can be acquired
- Space-saving design—at 12 x 19 x 13 in. (W x D x H), system fits easily on lab bench or in cell culture hood
- Network-capable—can be connected to local wireless network for seamless data transfer to OneDrive™ or Thermo Fisher Connect accounts. Alternatively, system offers on-board storage or export to USB.
- Flexibility—choose from wide selection of EVOS-compatible objectives (1.25–60X) to best fit application and image quality needs.
Dynabeads Magnetic Beads
Dynabeads products center around the goals to provide reliable and reproducible results for the purification and analysis of cells, proteins, exosomes, DNA, RNA, and other molecules. Dynabeads magnetic beads and products deliver highly cited results in the research industry across multiple application areas. Meanwhile, Thermo Fisher Scientific provides exceptional service and support backed by over 30+ years of knowledge and expertise.
What are Dynabeads magnetic beads?
- Dynabeads magnetic beads are superparamagnetic particles—they exhibit magnetic properties when placed in a magnetic field but retain no residual magnetism once removed from the magnetic field
- Unique batch-to-batch reproducibility secures reproducible, quality results
- Spherical shape and defined surface chemistry minimizes chemical agglutination and non-specific binding
- Polymer shell on each bead protects your target from toxic exposure to iron
- Bioreactive molecules can be absorbed or coupled to their surface, and used to separate biological materials (cells, proteins, nucleic acids, etc.)
Dynabeads magnetic beads come in several sizes and different surface functionalities for use in a wide variety of applications. Some beads are precoupled with a biomolecule (ligand). The ligand can be an antibody, protein or antigen, DNA/RNA probe, or any other molecule with an affinity for the desired target.
Mr. Frosty™ Freezing Container
Freeze cells in tubes from 1 to 5 mL using the Thermo Scientific™ Mr. Frosty™ Freezing Container. The simple-to-use system is designed to achieve a rate of cooling very close to -1°C/minute, the optimal rate for cell preservation. It holds 12 to 18 tubes, and a screw top lid secures samples inside in the event of accidental container tipping.
Features:
- Holds 12 to 18 tubes and has profile height of 86 or 151mm (3-7/16 to 5-5/16 in.), which saves precious freezer space during the cooling process
- Screw top lid secures samples inside in the event of accidental container tipping
- Eliminates unreliable, improvised foam container devices
- Easy to use—requires only 100% isopropyl alcohol and a mechanical freezer
- No contact with the alcohol: no wicking, no label removal and no compromise of tube hand printing
Ordering Information:
Containers have 4.75 in. diameter (120mm) closure
Requires:
Isopropyl alcohol and a mechanical freezer
Gibco Cryopreservation Media
Freezing down cells guards against unplanned mutations, senescence, and contamination. While unprotected cell freezing is ordinarily lethal, cell freezing with cryopreservation media helps protect cells for long-term viability as well as minimizes freezing injury for an intact cell supply on your schedule.
View protocols for cell freezing and thawing for more information.
- Recovery Cell Culture Freezing Medium
A complete, ready-to-use freezing medium, the Recovery medium formulation avoids messy mixing. Using an optimized version of the classic DMEM, serum, and DMSO, Recovery cell freezing medium is ready to go when you are. When treated with Recovery medium, cells experience improved recovery and viability post-thaw.
- Synth-a-Freeze Cryopreservation Medium
Formulated without antibiotics, antimycotics, hormones, growth factors, serum or proteins, Synth-a-Freeze medium is defined and ready-to-use. Synth-a-Freeze medium is buffered with HEPES and sodium bicarbonate and designed for use with primary mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells, and mesenchymal stem cells.
- PSC Cryopreservation Kit
The PSC Cryopreservation Kit contains ready-to-use, xeno-free cryopreservation medium as well as a bottle of RevitaCell Supplement (100X). Together, these reagents minimize cell viability loss and unwanted cell differentiation while maximizing post-thaw recovery. Applications include pluripotent stem cells cultured in media systems, such as Essential 8 and StemPro hESC SFM, as well as other commercially available formulations. In addition, this kit can also be used to cryopreserve and recover peripheral blood mononuclear cells (PBMCs).
- RevitaCell supplement
Containing Rho-associated protein kinase (ROCK) inhibitors and antioxidant properties, the RevitaCell supplement typically recovers pluripotent stem cells at 40,000 viable cells/cm2, and significantly reduces the average post-thaw recovery time of most cryopreservation solutions. In addition to pluripotent stem cells, RevitaCell supplements can also be utilized with primary cells including neurons, human epidermal keratinocytes, and human corneal epithelial cells.
Cell Culture Imaging Dishes, Plates, Slides and Coverglass
The Thermo Scientific Nunc Cell Culture Imaging Products offer comprehensive solutions with flexibility and consistency to meet your needs in cell imaging applications, including fluorescence microscopy, phase contrast microscopy, confocal microscopy, live cell imaging, differential interference contrast (DIC) microscopy, and fluorescence in situ hybridization (FISH).
Featuring removable medium chambers, the Thermo Scientific Nunc Lab-Tek and Lab-Tek II Chamber Slide systems allow you to seed, incubate, fix, stain and image on a single microscope slide, saving time and labor in the immunohistochemistry work.
Thermo Scientific Nunc Lab-Tek and Lab-Tek II Chambered Coverglasses feature chambers mounted to coverslips to provide optimal characteristics required for high magnification, confocal, and live imaging analysis.
With minimum background noise, the Thermo Scientific Nunc 96- and 384-well Optical Bottom Plates offer a wide selection of colors and surfaces ideal for cell-based fluorescence and luminescence assays.
Thermo Scientific Nunc glass bottom dishes combine the convenience of standard 35 mm cell culture dishes with the optical characteristics of coverslips to solve commonly encountered issues during live-cell imaging, such as medium evaporation and contamination.
Thermo Scientific Nunc Flasks on Slides provide suitable solutions for cell karyotyping using single cell autoradiography or single-cell immunofluorescence.
Cell Culture Plates with Inserts
Cell culture inserts are designed to be used in concert with carrier plates. These inserts can be slotted in multiple positions, allowing you to adjust well growth surface height, as well as the resulting media volume required for such a height.
This customizability allows you to optimize for gas exchange, oxygen tension, pH, and growth factor availability. Different insert pore sizes are available depending on application. Navigate to the associated guide using the button below.
- Add the ability to adjust your inserts to various heights within each well; our carrier plate fits perfectly into our 12- or 24-well plates
- Optimize the position of each porous membrane insert and volume of media required for your application
- Save time when feeding cells; with the carrier plate pipetting into each well separately is no longer necessary
- Conveniently adjust media volumes to extend feeding times (great for weekends)
Gibco LV-MAX Lentiviral Production System
Lentiviral vector production suspension solutions, for research and therapy:
The Gibco LV-MAX Lentiviral Production system is the first optimized system that provides all the high-quality components you need in a chemically defined, serum-free environment.
- High titers—greater than 1 x 108 TU/mL (unconcentrated)
- Research-grade and CTS options—seamlessly transition from discovery to commercial production
Optimized and fully integrated system includes:
HEK 293–derived suspension cells, serum-free media, proprietary transfection reagent, supplement, and our novel enhancer. Gibco HEK 293 derived viral production cells have been optimized for viral production in suspension culture.
Nunc™& Nalgene™ Cryogenic Tubes
Reliable, quality storage tubes that fit your workflow
Our portfolio of sample storage tubes provides reliable protection and security of cells, biological, genomic and compound materials at ultra-low or cryogenic temperatures. Our tubes are designed to ensure sample integrity over time and engineered with a focus on performance, quality and scalability within your lab’s workflow.
Sample storage tubes from Thermo Scientific are available in a wide variety of sizes, capping and storage formats, and with integrated sample identification options to support both manual and automated processing needs while emphasizing the safety and traceability of your valuable samples.
