Primary cell culture – Choose your cell type
Organoid and spheroid 3D cell culture using primary cells
Cells are the foundation of any 3D model, and cell selection is a critical first step in the generation of organoids and spheroids. The ultimate goal is to accurately represent the desired biology by choosing a cell and the reagents needed to create the growth conditions that will lead to an accurate 3D model, but there are many factors to consider. For example, primary cells are typically more “mature” and have an epigenetic memory, but typically don’t live as long and are more difficult to edit.