To keep cells in a more native state in vitro, it helps to tailor their environment by including extracellular matrix (ECM) components that emulate what the cells experience in vivo. By including a relevant cell culture matrix, you can better recapitulate the in vivo environment in your cell culture model. Gibco extracellular matrices, scaffolds, and proteins provide in vivo-like morphology and physiologically relevant cellular environments for more realistic cell culture models.

• Geltrex  products are reduced growth factor basement membrane extracts derived from mouse Engelbreth-Holm-Swarm (EHS) tumors.
• Laminin is the major glycoprotein in basement membranes. Mouse laminin facilitates the attachment and expansion of ESCs, iPSCs, and NSCs.
• Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). This cell culture matrix was designed for use with Essential 8 Medium and has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines.
• Collagen is the most widely used extracellular matrix protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.
• Poly-D-lysine (PDL) is a synthetic ECM widely used to promote cell adhesion of a variety of cell types. PDL is also ideal for finicky cells, such as primary and PSC-derived neurons.

Below you will find overview of the cell lines, culture conditions, and analysis techniques being used in the transition from 2D to 3D cell models.