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Gibco Cryopreservation Media
Freezing down cells guards against unplanned mutations, senescence, and contamination. While unprotected cell freezing is ordinarily lethal, cell freezing with cryopreservation media helps protect cells for long-term viability as well as minimizes freezing injury for an intact cell supply on your schedule.
View protocols for cell freezing and thawing for more information.
- Recovery Cell Culture Freezing Medium
A complete, ready-to-use freezing medium, the Recovery medium formulation avoids messy mixing. Using an optimized version of the classic DMEM, serum, and DMSO, Recovery cell freezing medium is ready to go when you are. When treated with Recovery medium, cells experience improved recovery and viability post-thaw.
- Synth-a-Freeze Cryopreservation Medium
Formulated without antibiotics, antimycotics, hormones, growth factors, serum or proteins, Synth-a-Freeze medium is defined and ready-to-use. Synth-a-Freeze medium is buffered with HEPES and sodium bicarbonate and designed for use with primary mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells, and mesenchymal stem cells.
- PSC Cryopreservation Kit
The PSC Cryopreservation Kit contains ready-to-use, xeno-free cryopreservation medium as well as a bottle of RevitaCell Supplement (100X). Together, these reagents minimize cell viability loss and unwanted cell differentiation while maximizing post-thaw recovery. Applications include pluripotent stem cells cultured in media systems, such as Essential 8 and StemPro hESC SFM, as well as other commercially available formulations. In addition, this kit can also be used to cryopreserve and recover peripheral blood mononuclear cells (PBMCs).
- RevitaCell supplement
Containing Rho-associated protein kinase (ROCK) inhibitors and antioxidant properties, the RevitaCell supplement typically recovers pluripotent stem cells at 40,000 viable cells/cm2, and significantly reduces the average post-thaw recovery time of most cryopreservation solutions. In addition to pluripotent stem cells, RevitaCell supplements can also be utilized with primary cells including neurons, human epidermal keratinocytes, and human corneal epithelial cells.