Primary Neuronal and Neural Cell Culture
Applications for the B-27 Plus Neuronal Cell Culture System
B-27 Plus supplement and Neurobasal Plus Medium come in the same formats and will work in the same protocols as the original products. Thermo Fisher Scientific recommends using the Plus versions of medium and supplement in the following applications:
Application | Recommended supplement(s) | Recommended basal medium |
---|---|---|
Maintenance/maturation of pre-natal/fetal primary neurons | B-27 Plus supplement | Neurobasal Plus Medium |
Maintenance/maturation of stem cell–derived neurons | B-27 Plus supplement and CultureOne Supplement |
Neurobasal Plus Medium |
Electrophysiology studies | B-27 Plus supplement | Neurobasal Plus Medium |
Maintenance/maturation of postnatal and adult brain neurons | B-27 Plus supplement | Neurobasal-A Medium |
Studies of oxidative stress/damage, apoptosis, or where free radical damage to neurons occurs | B-27 supplement minus Antioxidants | Neurobasal Plus Medium |