Luminex Instruments

The Luminex family of systems encompasses four instrument models, each offering distinct multiplexing capabilities, throughput, and read times. These Luminex instruments operate on the quantitative fluorescent flow cytometry principle, leveraging the xMAP magnetic bead technology to detect small quantities of analytes. Used in combination with our ProcartaPlex multiplex assays for protein quantitation or QuantiGene Plex assays for gene expression analysis, the Luminex platform delivers a rapid and cost-effective immunoassay profiling tool. This synergy between Luminex instruments and our advanced multiplex assays enhances efficiency and precision in the detection and quantification of biomolecules.

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Description

Which Luminex model is right for you?

xMAP INTELLIFLEX
DR-SE
 System
xMAP INTELLIFLEX
System
FLEXMAP 3D

System

Luminex 200

System

Double the data with the second reporter channel Compact footprint with our broadest dynamic range Fast read-time and high-throughput Fast and robust platform for up to 100 analytes
Performance
Multiplex capacity Up to 500

Up to 2 parameters per analytes

Up to 500 Up to 500 Up to 100

(80 on MagPlex)

Dynamic range ≥ 5.5 logs (RP1)

≥ 4.5 logs (RP2)

≥ 5.5 logs (RP1) ≥ 4.5 logs (RP1) ≥ 3.5 logs (RP1)
Read time 96-well in ~20 min

384-well in ~75 min

96-well in ~20 min

384-well in ~75 min

96-well in ~20 min

384-well in ~75 min

45 min (96-well)
Sensitivity (assay dependent) 0.06–1 pg/mL (Protein assays); 1,000 RNA copies per target/well (RNA assays)
Technical
Footprint including PC (linear bench space) 58.4 cm x 61 cm x 76 cm 58.4 cm x 61 cm x 76 cm 110 cm x 62 cm x 63 cm 115 cm x 60 cm x 50 cm
Weight 54.5 kg (120 lb) 54.5 kg (120 lb) 77.1 kg (170 lb) 49 kg (113 lb)
Optics Flow cytometry-based lasers, APDs, PMTs
Reporter laser/optics 532 nm (green) and 405 nm (violet) 532 nm (green) 532 nm (green) 532 nm (green)
Automation-compatible hardware Front and side eject Front eject only Front eject only Front eject only
Operational validation Calibration, verification, and fluidics
Application
Luminex assays Protein assays/nucleic acid assays (ProcartaPlex / QuantiGene Plex)
Sample volume 6.3–80 µL (assay dependent) 25–80 µL (assay dependent)
Largest available panel 80
Plate format 96-well or 384-well 96-well or 384-well 96-well or 384-well 96-well
Software
21 CFR compliance Yes
Automation Module Yes Yes Integration software Integration software
Analysis software Data analysis apps for Luminex assays on the
Thermo Fisher Connect Platform
xPONENT basic plus Thermo Fisher Connect Platform

biomarker, multiplex ELISA

Additional information
Manufacturer