Molecular Biology
No matter what your workflow requirements may be, our extensive range of molecular solutions is specifically designed to deliver high performance, save you time, and provide the reliability you need for successful PCR and cloning experiments.
We understand that every product you select plays a crucial role in advancing scientific research. That’s why we offer a diverse selection of solutions that are tailored for discovery, covering everything from sample preparation to reverse transcription, PCR, and cloning.
Our team of product experts and scientific support staff are always ready to assist you in considering important factors for choosing most fitting products for successful sample preparation, reverse transcription, PCR, electrophoresis, cloning, and gene synthesis.
PowerFlex™ Thermal Cycler, 3×32-well
Ultimate Performance, Compatibility, and Productivity for Routine and High-Throughput Molecular Workflows.
The Applied Biosystems PowerFlex Thermal Cycler is the ultimate day-to-day workhorse for standard research, academic, and routine testing laboratories, while delivering the premium performance required for high-throughput biotech and pharmaceutical pipelines. Engineered for maximum versatility, the PowerFlex system features a unique 3 x 32-well independent block format that allows individual researchers, technicians, or students to run completely distinct thermal protocols simultaneously on a single compact instrument. This eliminates laboratory scheduling bottlenecks and optimizes bench space without compromising on scientific precision. With an exceptional block ramp rate of up to 7.0°C/sec, a stringent temperature accuracy of ±0.15°C, and a superior well-to-well uniformity of <0.4°C (at 95°C), the system ensures absolute consistency for routine applications. Additionally, its redesigned architecture offers seamless compatibility with 96-well fully skirted, semi-skirted, and non-skirted PCR plates, making it fully ready for integration into automated liquid handling and robotic screening pipelines.
Key Features & Benefits
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Accelerated Thermal Performance: Reaches an impressive block ramp rate of up to 7.0°C/sec and a maximum sample ramp rate of 6.2°C/sec, significantly driving down total protocol run times and accelerating daily sample throughput.
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True Multi-User Protocol Flexibility: Equipped with a versatile 3 x 32-well block format (0.2 mL) with fully independent control, allowing up to three different researchers to configure and run completely distinct thermal profiles at the same time.
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Broad Consumable Compatibility: Upgraded block design provides out-of-the-box compatibility with a wide range of PCR plate types, including fully skirted, semi-skirted, and non-skirted 96-well formats, as well as 8-tube strips and individual tubes.
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Seamless Simulation Mode: Allows running protocols under the exact same conditions as legacy thermal cyclers, ensuring a smooth and rapid transition of established protocols from the ProFlex PCR System without requiring re-optimization.
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VeriFlex Block Technology: Utilizes multiple independent Peltier elements (featuring 3 temperature zones per 32-well block) to enable precise linear temperature control for fast and accurate PCR optimization.
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Intuitive Interface and Enlarged Screen: Features an adjustable 10.1-inch color TFT touchscreen (20% larger than the ProFlex model) with an intuitive interface for effortless, interactive programming.
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Onboard Smart Help Diagnostics: Includes built-in automated troubleshooting capabilities for fast and accurate resolution of technical questions, ensuring maximal instrument uptime.
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Secure Remote Access: Supported by the Thermo Fisher Connect Platform, enabling secure remote monitoring and access anytime, anywhere from a mobile device or desktop to design protocols, check run status, and download experimental reports.
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Compact, Space-Saving Footprint: Optimized hardware design delivers a 20% smaller footprint compared to the previous ProFlex platform, freeing up valuable laboratory bench space.
Applications The PowerFlex Thermal Cycler is purpose-built to support core molecular biology workflows across multiple research and industrial application fields such as standard cloning, genotyping, cloning sequencing, and mutagenesis workflows and advanced assay development and screening optimization within biotechnology and pharmaceutical R&D facilities.
iGlow™ Gel Documentation System
Flexible, Intuitive One-Touch Gel Imaging with Advanced Automated Workflow and Extended Support.
The Invitrogen iGlow Gel Documentation System is a flexible, highly intuitive imaging platform engineered to deliver rapid, easy nucleic acid and protein gel imaging across a diverse range of gel formats. Featuring an advanced ‘one-touch capture’ functionality, the system streamlines the image acquisition process, taking mere seconds to go from sample insertion to high-quality data capture. At its core, the system incorporates a sophisticated Windows-based computing platform that cleanly integrates hardware and software components, eliminating the need for external computing devices and freeing valuable bench space. Equipped with a high-resolution 5-megapixel scientific CMOS (sCMOS) detector and three distinct LED transilluminator light sources (blue, UV, and white), the iGlow enables precision imaging for a wide range of samples and stains. To maximize workflow efficiency and remove manual guesswork, the system features automatic tray recognition that senses exactly which sample tray is inserted and activates only the corresponding imaging mode. Furthermore, during capture, the intelligent software automatically detects sample intensity levels to calculate the ideal exposure time, ensuring consistent, publication-quality results. Providing ultimate peace of mind for laboratory operations, the entire system package includes all essential imaging trays, a UV safety shield, and is backed by a comprehensive, industry-leading two-year warranty.
Product Features
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One-Touch Image Capture System: Streamlined operation with single-touch activation enables rapid image acquisition and enhanced user efficiency for high-throughput laboratory workflows.
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Automated Tray Recognition Technology: Intelligent detection system automatically identifies sample trays upon insertion, removing manual steps, simplifying user experience, and reducing potential for user error.
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Integrated Touchscreen Interface: Built-in 12-inch touchscreen display provides intuitive navigation and standalone system control, driven by an onboard Windows-based computer platform.
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High Resolution 5-MP sCMOS Camera: Powerful scientific imaging sensor enables publication-quality image capture with superior clarity, contrast, and fine detail suitable for rigorous scientific documentation and analysis.
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Multi-Source Gel Excision Capability: Supports precision gel excision and band-cutting workflows utilizing white, blue, and UV light sources for versatile downstream sample preparation applications.
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Integrated Safety Detection System: Automated safety screen monitoring uses smart proximity sensors to ensure the UV shield remains safely in place during gel excision procedures, preventing potential user exposure.
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Automated Exposure Capture: System automatically measures sample intensity levels to calculate the perfect exposure time, removing guesswork and preventing band saturation.
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Comprehensive Data Transfer Options: Multiple connectivity solutions including Ethernet, Wi-Fi, and USB interfaces enable flexible data management, direct printing, and easy integration with network infrastructures.
Applications & Data Processing
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Multi-Stain Visualization: Precision imaging of fluorescently stained DNA/RNA agarose gels (Ethidium Bromide, SYBR Safe, gel stains) and colorimetric or fluorescent protein gels (Coomassie, silver stains).
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Onboard and Advanced Image Analysis: Perform basic adjustments directly on the standalone imager panel, with full data compatibility for the included iBright Analysis Software (iBA) to conduct advanced quantification and custom reporting across Mac, PC, or Cloud environments.
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Versatile Purification Workflows: Safe and accurate band excision under multiple light paths to support clean upstream sample validation for downstream cloning and sequencing pipelines.
Sample preparation – Nucleic Acid Isolation
Nucleic acid isolation is a crucial first step in the molecular biology workflow, whether you are isolating genomic DNA (gDNA) or RNA. Selecting nucleic acid purification products that are optimized to provide maximum yield, purity, and integrity from virtually any sample type and application is important for your research success.
We offer complete solution portfolio for sample extraction and preparation:
• Magnetic beads: 0.5–1.0 μm particles with a paramagnetic core and modified shell (e.g., Applied Biosystems™ MagMAX™ kits and Invitrogen™ Dynabeads™ magnetic beads) Samples are lysed in solution and allowed to bind nucleic acid to magnetic particles based on specific surface modifications. Application of an external magnetic field rapidly collects the particles. Rounds of release, wash, and recapture enable purification of the desired nucleic acid.
• Spin columns: Glass fiber, derivatized silica, or ion exchange membrane in column (e.g., Thermo Scientific™ GeneJET™ and Invitrogen™ PureLink™ kits) Samples are lysed and passed through the membrane using centrifugal or vacuum force. Wash and elution solutions are subsequently passed through the membrane, and the sample is collected into a tube by centrifugation.
• Organic extraction: Phenol-chloroform solution (e.g., Invitrogen™ DNAzol™ and TRIzol™ Reagents) After homogenizing the sample with TRIzol Reagent, chloroform is added, and the mixture separates into a clear upper aqueous layer containing RNA, an interphase layer, and a pink lower organic layer containing the DNA and protein. RNA is precipitated from the upper aqueous layer with isopropanol. DNA is precipitated from the interphase and organic layers with ethanol. Protein is precipitated from the phenol–ethanol supernatant with isopropanol.
KingFisher Automation
KingFisher purification instruments automate the extraction and purification of DNA, RNA, proteins, and cells. KingFisher purification instrument models serve applications ranging from SARS-CoV-2 and infectious diseases, extracting viral or pathogen nucleic acid, to isolating genomic DNA (gDNA) for genetic testing. Their broad functionality and customizable software feature additionally serve applications, such as FFPE and cell-free DNA (cfDNA) tissue and liquid biopsy, immunoprecipitation, peptide mapping, and cells and exosomes.
Reverse Transcription
Reverse transcription is the synthesis of complementary DNA (cDNA) from an RNA template using reverse transcriptase enzymes. The cDNA can be used as a template for PCR amplification, cDNA library construction, RNA sequencing, and more. Selecting the right reverse transcriptase is critical to detecting low-abundance RNAs in a sample and obtaining high yields of full-length cDNA.
Choose from a variety of reverse transcriptase stand-alone enzymes, master mixes, cDNA synthesis kits, primers, and dNTP solutions to suit your application. Invitrogen SuperScript IV products aid in exceptional cDNA synthesis from any type of template RNA.
Click “Learn more” for reverse transcription reagent selection guide.
PCR Reagents and Enzymes
Choose from a variety of PCR enzymes and reagents for your applications, with the flexibility needed to perform your experiments.
With PCR enzymes you know and trust, such as, Applied Biosystems AmpliTaq and AmpliTaq Gold, Invitrogen Platinum II Taq , and Platinum SuperFi II DNA polymerases, we have what it takes for successful PCR.
Click “Learn more” for interactive PCR Enzymes Selection Tool.
Molecular Cloning Essentials
Innovative products for the molecular cloning workflow:
The molecular cloning workflow requires multiple steps to obtain high-quality cloned DNA for your downstream applications. Help advance your molecular cloning workflow at every step and accelerate construction of recombinant clones. Our wide range of cloning solutions address needs from routine to specialty applications, helping you find optimal reagents to achieve fast results with greater confidence.
Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery, among the reputable solution: GeneArt Gibson Assembly Cloning; Gateway Cloning Technology; TOPO PCR Cloning and more.
Click on each step to explore the molecular cloning workflow and select products for your downstream applications that fit your research.
MiniAmp Thermal Cyclers: Compact Size, Elevated Routine PCR
Our thermocyclers are designed for reliability, accuracy, and user friendliness to fit scientists’ needs. Applied Biosystems PCR instruments are equipped with:
• VeriFlex temperature control technology with segmented blocks that allow for precise temperature control.
• Thermal Cycler Fleet Control Software—manage multiple instruments with the help of optional software to help enhance productivity of PCR machines with this secure remote access feature.
Backed by the proven reliability of Applied Biosystems technology the MiniAmp Plus Thermal Cycler and the MiniAmp Thermal Cycler were designed for routine PCR. With their compact design, just 19 cm wide, and entry-level price, MiniAmp Thermal Cyclers are perfect for every lab bench.
The MiniAmp Thermal Cycler has an isothermal block, while the MiniAmp Plus Thermal Cycler offers an upgrade to a VeriFlex Block for PCR optimization.
SimpliAmp Thermal Cycler: Ultimate Ease of Use and Simplicity
Our thermocyclers are designed for reliability, accuracy, and user friendliness to fit scientists’ needs. Applied Biosystems PCR instruments are equipped with:
• VeriFlex temperature control technology with segmented blocks that allow for precise temperature control.
• Thermal Cycler Fleet Control Software—manage multiple instruments with the help of optional software to help enhance productivity of PCR machines with this secure remote access feature.
The Applied Biosystems SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow. Innovations like a responsive color touch screen and Applied Biosystems VeriFlex temperature control technology enable simple, accurate optimization. And now you can access your SimpliAmp cycler with Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.
ProFlex PCR System: Ultimate Flexibility in a High-Throughput PCR System
The ProFlex system combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.
The ProFlex 3 x 32-well PCR System allows you to:
• Run three experiments at once with the 3 x 32-well, 0.2-mL block
• Interchange the 3 x 32-well block with another block type if throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate old instrument with Thermal Simulation modes
• Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks
VeritiPro Thermal Cycler: Better-Than-Gradient PCR Optimization
The Applied Biosystems VeritiPro Thermal Cycler delivers proven reliability with advanced temperature control technology and connectivity. Take advantage of next-level PCR optimization with the precision offered by VeriFlex block technology. Connect to the cloud-enabled VeritiPro Thermal Cycler remotely. Conveniently design and securely upload your methods, monitor runs, and check instrument availability from any mobile device or desktop computer using Connect, our cloud-based platform.
The VeritiPro Thermal Cycler comes with two options:
1. 96-well fixed block with 6 VeriFlex zones for user-independent temperature control
2. 384-well fixed and isothermal block
Gradient temperature control is a feature of thermal cyclers to help primer annealing in PCR. However, thermal cyclers equipped with “better-than-gradient” technology are alternatives that help improve temperature control for primer annealing. These metal blocks often come with three or more segmented blocks, each with separate heating and cooling elements. VeritiPro Thermal Cycler is equipped with “better-than-gradient” technology, namely Veriflex Blocks.
E-Gel™ Power Snap Lite Electrophoresis System
Streamlined, Precast Nucleic Acid Gel Electrophoresis for Fast and Consistent Everyday Analysis.
The Invitrogen E-Gel Power Snap Lite Electrophoresis System is a horizontal gel electrophoresis system expertly optimized for rapid DNA and RNA verification within everyday molecular biology workflows. Designed to streamline routine nucleic acid analysis, this compact platform delivers efficient, high-quality results without the multi-step complexity, manual labor, and mess typically associated with conventional horizontal electrophoresis. By utilizing advanced precast agarose gel technology, the system elegantly resolves a major laboratory bottleneck by completely eliminating the time-consuming tasks of weighing agarose, melting polymers, and preparing running buffers. The system introduces a redesigned, highly intuitive touchscreen interface equipped with preset protocols, guaranteeing fast, reproducible, and consistent separation runs regardless of user experience. Its modern, compact architecture successfully overcomes space constraints on crowded or shared laboratory benches, providing busy facilities with an uncompromised, ready-to-run solution for routine sample screening.
Key Features & Benefits
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Streamlined Workflow: Optimized specifically for quick DNA and RNA verification, accelerating the routine check stages within everyday genomics pipelines.
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Precast Convenience: Operates utilizing convenient precast E-Gel agarose gels, entirely removing the need for manual gel pouring and chemical buffer preparation.
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Redesigned User Interface: Features an updated touchscreen interface with integrated preset protocols to drive fast, consistent, and user-friendly operation.
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Compact Bench Footprint: A smart, small-footprint design that fits effortlessly into space-conscious laboratories and high-traffic shared laboratory benches.
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Greener Product Choice: Engineered with a more sustainable instrument shell that contains at least 10% postconsumer recycled content to support eco-conscious laboratory initiatives.
Applications
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Rapid post-PCR verification of DNA and RNA fragments within general molecular biology protocols.
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Routine horizontal gel electrophoresis screening without the infrastructure required for traditional hand-poured gel systems.
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High-frequency workflow checks on space-constrained shared resource lab benches.
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