PRODUCTS
Sample preparation – Nucleic Acid Isolation
Nucleic acid isolation is a crucial first step in the molecular biology workflow, whether you are isolating genomic DNA (gDNA) or RNA. Selecting nucleic acid purification products that are optimized to provide maximum yield, purity, and integrity from virtually any sample type and application is important for your research success.
We offer complete solution portfolio for sample extraction and preparation:
• Magnetic beads: 0.5–1.0 μm particles with a paramagnetic core and modified shell (e.g., Applied Biosystems™ MagMAX™ kits and Invitrogen™ Dynabeads™ magnetic beads) Samples are lysed in solution and allowed to bind nucleic acid to magnetic particles based on specific surface modifications. Application of an external magnetic field rapidly collects the particles. Rounds of release, wash, and recapture enable purification of the desired nucleic acid.
• Spin columns: Glass fiber, derivatized silica, or ion exchange membrane in column (e.g., Thermo Scientific™ GeneJET™ and Invitrogen™ PureLink™ kits) Samples are lysed and passed through the membrane using centrifugal or vacuum force. Wash and elution solutions are subsequently passed through the membrane, and the sample is collected into a tube by centrifugation.
• Organic extraction: Phenol-chloroform solution (e.g., Invitrogen™ DNAzol™ and TRIzol™ Reagents) After homogenizing the sample with TRIzol Reagent, chloroform is added, and the mixture separates into a clear upper aqueous layer containing RNA, an interphase layer, and a pink lower organic layer containing the DNA and protein. RNA is precipitated from the upper aqueous layer with isopropanol. DNA is precipitated from the interphase and organic layers with ethanol. Protein is precipitated from the phenol–ethanol supernatant with isopropanol.
KingFisher Automation
KingFisher purification instruments automate the extraction and purification of DNA, RNA, proteins, and cells. KingFisher purification instrument models serve applications ranging from SARS-CoV-2 and infectious diseases, extracting viral or pathogen nucleic acid, to isolating genomic DNA (gDNA) for genetic testing. Their broad functionality and customizable software feature additionally serve applications, such as FFPE and cell-free DNA (cfDNA) tissue and liquid biopsy, immunoprecipitation, peptide mapping, and cells and exosomes.
Reverse Transcription
Reverse transcription is the synthesis of complementary DNA (cDNA) from an RNA template using reverse transcriptase enzymes. The cDNA can be used as a template for PCR amplification, cDNA library construction, RNA sequencing, and more. Selecting the right reverse transcriptase is critical to detecting low-abundance RNAs in a sample and obtaining high yields of full-length cDNA.
Choose from a variety of reverse transcriptase stand-alone enzymes, master mixes, cDNA synthesis kits, primers, and dNTP solutions to suit your application. Invitrogen SuperScript IV products aid in exceptional cDNA synthesis from any type of template RNA.
Click “Learn more” for reverse transcription reagent selection guide.
PCR Reagents and Enzymes
Choose from a variety of PCR enzymes and reagents for your applications, with the flexibility needed to perform your experiments.
With PCR enzymes you know and trust, such as, Applied Biosystems AmpliTaq and AmpliTaq Gold, Invitrogen Platinum II Taq , and Platinum SuperFi II DNA polymerases, we have what it takes for successful PCR.
Click “Learn more” for interactive PCR Enzymes Selection Tool.
Molecular Cloning Essentials
Innovative products for the molecular cloning workflow:
The molecular cloning workflow requires multiple steps to obtain high-quality cloned DNA for your downstream applications. Help advance your molecular cloning workflow at every step and accelerate construction of recombinant clones. Our wide range of cloning solutions address needs from routine to specialty applications, helping you find optimal reagents to achieve fast results with greater confidence.
Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery, among the reputable solution: GeneArt Gibson Assembly Cloning; Gateway Cloning Technology; TOPO PCR Cloning and more.
Click on each step to explore the molecular cloning workflow and select products for your downstream applications that fit your research.
MiniAmp Thermal Cyclers: Compact Size, Elevated Routine PCR
Our thermocyclers are designed for reliability, accuracy, and user friendliness to fit scientists’ needs. Applied Biosystems PCR instruments are equipped with:
• VeriFlex temperature control technology with segmented blocks that allow for precise temperature control.
• Thermal Cycler Fleet Control Software—manage multiple instruments with the help of optional software to help enhance productivity of PCR machines with this secure remote access feature.
Backed by the proven reliability of Applied Biosystems technology the MiniAmp Plus Thermal Cycler and the MiniAmp Thermal Cycler were designed for routine PCR. With their compact design, just 19 cm wide, and entry-level price, MiniAmp Thermal Cyclers are perfect for every lab bench.
The MiniAmp Thermal Cycler has an isothermal block, while the MiniAmp Plus Thermal Cycler offers an upgrade to a VeriFlex Block for PCR optimization.
SimpliAmp Thermal Cycler: Ultimate Ease of Use and Simplicity
Our thermocyclers are designed for reliability, accuracy, and user friendliness to fit scientists’ needs. Applied Biosystems PCR instruments are equipped with:
• VeriFlex temperature control technology with segmented blocks that allow for precise temperature control.
• Thermal Cycler Fleet Control Software—manage multiple instruments with the help of optional software to help enhance productivity of PCR machines with this secure remote access feature.
The Applied Biosystems SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow. Innovations like a responsive color touch screen and Applied Biosystems VeriFlex temperature control technology enable simple, accurate optimization. And now you can access your SimpliAmp cycler with Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.
ProFlex PCR System: Ultimate Flexibility in a High-Throughput PCR System
The ProFlex system combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.
The ProFlex 3 x 32-well PCR System allows you to:
• Run three experiments at once with the 3 x 32-well, 0.2-mL block
• Interchange the 3 x 32-well block with another block type if throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate old instrument with Thermal Simulation modes
• Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks
VeritiPro Thermal Cycler: Better-Than-Gradient PCR Optimization
The Applied Biosystems VeritiPro Thermal Cycler delivers proven reliability with advanced temperature control technology and connectivity. Take advantage of next-level PCR optimization with the precision offered by VeriFlex block technology. Connect to the cloud-enabled VeritiPro Thermal Cycler remotely. Conveniently design and securely upload your methods, monitor runs, and check instrument availability from any mobile device or desktop computer using Connect, our cloud-based platform.
The VeritiPro Thermal Cycler comes with two options:
1. 96-well fixed block with 6 VeriFlex zones for user-independent temperature control
2. 384-well fixed and isothermal block
Gradient temperature control is a feature of thermal cyclers to help primer annealing in PCR. However, thermal cyclers equipped with “better-than-gradient” technology are alternatives that help improve temperature control for primer annealing. These metal blocks often come with three or more segmented blocks, each with separate heating and cooling elements. VeritiPro Thermal Cycler is equipped with “better-than-gradient” technology, namely Veriflex Blocks.
E-Gel™ Power Snap Lite Electrophoresis System
Streamlined, Precast Nucleic Acid Gel Electrophoresis for Fast and Consistent Everyday Analysis.
The Invitrogen E-Gel Power Snap Lite Electrophoresis System is a horizontal gel electrophoresis system expertly optimized for rapid DNA and RNA verification within everyday molecular biology workflows. Designed to streamline routine nucleic acid analysis, this compact platform delivers efficient, high-quality results without the multi-step complexity, manual labor, and mess typically associated with conventional horizontal electrophoresis. By utilizing advanced precast agarose gel technology, the system elegantly resolves a major laboratory bottleneck by completely eliminating the time-consuming tasks of weighing agarose, melting polymers, and preparing running buffers. The system introduces a redesigned, highly intuitive touchscreen interface equipped with preset protocols, guaranteeing fast, reproducible, and consistent separation runs regardless of user experience. Its modern, compact architecture successfully overcomes space constraints on crowded or shared laboratory benches, providing busy facilities with an uncompromised, ready-to-run solution for routine sample screening.
Key Features & Benefits
-
Streamlined Workflow: Optimized specifically for quick DNA and RNA verification, accelerating the routine check stages within everyday genomics pipelines.
-
Precast Convenience: Operates utilizing convenient precast E-Gel agarose gels, entirely removing the need for manual gel pouring and chemical buffer preparation.
-
Redesigned User Interface: Features an updated touchscreen interface with integrated preset protocols to drive fast, consistent, and user-friendly operation.
-
Compact Bench Footprint: A smart, small-footprint design that fits effortlessly into space-conscious laboratories and high-traffic shared laboratory benches.
-
Greener Product Choice: Engineered with a more sustainable instrument shell that contains at least 10% postconsumer recycled content to support eco-conscious laboratory initiatives.
Applications
-
Rapid post-PCR verification of DNA and RNA fragments within general molecular biology protocols.
-
Routine horizontal gel electrophoresis screening without the infrastructure required for traditional hand-poured gel systems.
-
High-frequency workflow checks on space-constrained shared resource lab benches.
Contact us for DEMO
E-Gel™ Power Snap Plus Electrophoresis Systems
Small, fast, and powerful-
The all-in-one E-Gel Power Snap Plus Electrophoresis System provides everything you need for DNA gel electrophoresis and gel visualization in a small lightweight benchtop system. The system includes an electrophoresis power supply, blue-light transilluminator, amber filter, and adapter for 11- to 22-well E-Gel agarose gels. This enables gel separation and real-time sample tracking on E-Gel agarose gels pre-stained with SYBR Safe or SYBR Gold II DNA stains. The device arrives with pre-programmed protocols for each type of compatible E-Gel agarose gel.
• Fast and safe workflow using Invitrogen™ E-Gel™ technology, Invitrogen™ SYBR™ Safe DNA Gel Stain, and a blue-light transilluminator
• Convenience of gel-running and documentation on the same small device
• Easy analysis on both the device and on the Thermo Fisher™ Connect Platform with Invitrogen™ iBright™ image analysis software
Contact us for DEMO
Owl Electrophoresis Systems
Perform agarose gel electrophoresis of nucleic acids using tanks, combs, power supplies, and other accessories of Thermo Scientific™ Owl™ Horizontal Gel Electrophoresis Systems. Choose among the following format series: large (A1-A6), micro (C2-S), wide (D2-D4), and mini (EasyCast™ B1-B3).
