Cell Culture essentials
Gibco Cell Culture Media
Cell culture medium is vital to your culture environment as it provides components (e.g., nutrients, growth factors) necessary to support cell growth and function. In addition, media can be formulated to regulate environmental conditions in cell culture, including osmotic pressure and pH. A variety of cell culture media formulations have been developed to support a wide range of cell types and experimental applications.
Time-tested and trusted, our Gibco cell culture media line includes products designed to support the growth and maintenance of a variety of mammalian cells and cell lines. We offer ready-to-use cell culture media as well as powdered and concentrated liquid formulations to fit your experimental setup and budget. For your scale-up needs, Gibco Cell Culture for Bioprocessing provides end-to-end products, process development and customization services to help maximize productivity while reducing costs and time to market.
Click “Learn more” for interactive selection tool to choose from all options including classical and innovative media formulations.
Cell culture reagents and supplements
Designed to deliver reproducibility, Gibco cell culture reagents and suppliments are infused with quality, customer-focused innovation, and service excellence from beginning to end. From the most basic formulations to the newest innovations, Gibco products offer exceptional quality, consistency, and performance—for results that you can count on every day.
Cell Culture Reagents and supplements portfolio include GIBCO’s Antibiotics, Essential Amino Acids and GlutaMAX, Balanced Salt Solutions, Cell Freezing Media, TrypLE Enzymes, Cell Dissociation and Trypsin for Cell Culture, Growth Factors & Cytokines, Water For Injection and more.
Click “Learn more” to browse our broad portfolio essential culture products.
Fetal bovine serum (FBS)
Fetal bovine serum (FBS) offers essential nutrients and growth factors for the maintenance and growth of cultured cells. Cell-based applications are an integral part of basic research, biochemical studies, vaccine development, and drug discovery to name a few. FBS is the most used serum in cell-based applications as it provides the most robust culture system for the widest range of cell types.
Since 1962, Gibco FBS products have been a global market leader. Through Thermo Fisher Scientific’s unwavering commitment to quality, scientists using Gibco FBS have been provided with consistent reliability, service, value, and innovation.
Click “Learn more” and discover the performance differences that Gibco Fetal Bovine Serum can deliver for your research and production. You can also contact us and request a sample.
PeproTech recombinant proteins
Recombinant cytokines, chemokines, and other RUO proteins
Most PeproTech recombinant proteins are based on native sequences from human, mouse, and rat, and are produced in bacterial, mammalian, or insect cells. Many PeproTech E. coli–produced proteins are animal origin–free as they are made without using animal-derived cell culture components or purification resins.
The PeproTech product line includes RUO recombinant cytokines important for research in:
- Stem cells
- 3D cell culture models
- Cancer cell biology
- Neurobiology
- Immunology
- Cardiovascular
PeproTech recombinant cytokines and proteins help life science, biotech, and pharma companies achieve success in their endeavors to discover new biological insights and develop cutting-edge cell and gene therapies.
Because every step of the production process is expertly managed within, PeproTech recombinant cytokines and growth factors are trusted for quality, consistency, and reliability as well as their ability to support the RUO-to-GMP transition.
Cell Lines & Primary Cells
We offer a variety of primary cultures and established cell lines, reagents, media, sera, and growth factors for your cell culture experiments.
Extracellular Matrices
To keep cells in a more native state in vitro, it helps to tailor their environment by including extracellular matrix (ECM) components that emulate what the cells experience in vivo. By including a relevant cell culture matrix, you can better recapitulate the in vivo environment in your cell culture model. Gibco extracellular matrices, scaffolds, and proteins provide in vivo-like morphology and physiologically relevant cellular environments for more realistic cell culture models.
- Geltrex products are reduced growth factor basement membrane extracts derived from mouse Engelbreth-Holm-Swarm (EHS) tumors.
- Laminin is the major glycoprotein in basement membranes. Mouse laminin facilitates the attachment and expansion of ESCs, iPSCs, and NSCs.
- Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). This cell culture matrix was designed for use with Essential 8 Medium and has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines.
- Collagen is the most widely used extracellular matrix protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.
- Poly-D-lysine (PDL) is a synthetic ECM widely used to promote cell adhesion of a variety of cell types. PDL is also ideal for finicky cells, such as primary and PSC-derived neurons.
Below you will find overview of the cell lines, culture conditions, and analysis techniques being used in the transition from 2D to 3D cell models.
Dynabeads Magnetic Beads
Dynabeads products center around the goals to provide reliable and reproducible results for the purification and analysis of cells, proteins, exosomes, DNA, RNA, and other molecules. Dynabeads magnetic beads and products deliver highly cited results in the research industry across multiple application areas. Meanwhile, Thermo Fisher Scientific provides exceptional service and support backed by over 30+ years of knowledge and expertise.
What are Dynabeads magnetic beads?
- Dynabeads magnetic beads are superparamagnetic particles—they exhibit magnetic properties when placed in a magnetic field but retain no residual magnetism once removed from the magnetic field
- Unique batch-to-batch reproducibility secures reproducible, quality results
- Spherical shape and defined surface chemistry minimizes chemical agglutination and non-specific binding
- Polymer shell on each bead protects your target from toxic exposure to iron
- Bioreactive molecules can be absorbed or coupled to their surface, and used to separate biological materials (cells, proteins, nucleic acids, etc.)
Dynabeads magnetic beads come in several sizes and different surface functionalities for use in a wide variety of applications. Some beads are precoupled with a biomolecule (ligand). The ligand can be an antibody, protein or antigen, DNA/RNA probe, or any other molecule with an affinity for the desired target.
Gibco Cryopreservation Media
Freezing down cells guards against unplanned mutations, senescence, and contamination. While unprotected cell freezing is ordinarily lethal, cell freezing with cryopreservation media helps protect cells for long-term viability as well as minimizes freezing injury for an intact cell supply on your schedule.
View protocols for cell freezing and thawing for more information.
- Recovery Cell Culture Freezing Medium
A complete, ready-to-use freezing medium, the Recovery medium formulation avoids messy mixing. Using an optimized version of the classic DMEM, serum, and DMSO, Recovery cell freezing medium is ready to go when you are. When treated with Recovery medium, cells experience improved recovery and viability post-thaw.
- Synth-a-Freeze Cryopreservation Medium
Formulated without antibiotics, antimycotics, hormones, growth factors, serum or proteins, Synth-a-Freeze medium is defined and ready-to-use. Synth-a-Freeze medium is buffered with HEPES and sodium bicarbonate and designed for use with primary mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells, and mesenchymal stem cells.
- PSC Cryopreservation Kit
The PSC Cryopreservation Kit contains ready-to-use, xeno-free cryopreservation medium as well as a bottle of RevitaCell Supplement (100X). Together, these reagents minimize cell viability loss and unwanted cell differentiation while maximizing post-thaw recovery. Applications include pluripotent stem cells cultured in media systems, such as Essential 8 and StemPro hESC SFM, as well as other commercially available formulations. In addition, this kit can also be used to cryopreserve and recover peripheral blood mononuclear cells (PBMCs).
- RevitaCell supplement
Containing Rho-associated protein kinase (ROCK) inhibitors and antioxidant properties, the RevitaCell supplement typically recovers pluripotent stem cells at 40,000 viable cells/cm2, and significantly reduces the average post-thaw recovery time of most cryopreservation solutions. In addition to pluripotent stem cells, RevitaCell supplements can also be utilized with primary cells including neurons, human epidermal keratinocytes, and human corneal epithelial cells.
Gibco LV-MAX Lentiviral Production System
Lentiviral vector production suspension solutions, for research and therapy:
The Gibco LV-MAX Lentiviral Production system is the first optimized system that provides all the high-quality components you need in a chemically defined, serum-free environment.
- High titers—greater than 1 x 108 TU/mL (unconcentrated)
- Research-grade and CTS options—seamlessly transition from discovery to commercial production
Optimized and fully integrated system includes:
HEK 293–derived suspension cells, serum-free media, proprietary transfection reagent, supplement, and our novel enhancer. Gibco HEK 293 derived viral production cells have been optimized for viral production in suspension culture.
