QUBIT – Fluorescence RNA/DNA Quantification

DNA, RNA, and protein quantification for a single or multiple samples

Qubit Fluorometers detect fluorescent dyes specifically bound to the target molecule. With optimized Qubit Assays, they can distinguish dsDNA from ssDNA or intact from degraded RNA, even in extremely small amounts or in the presence of contaminants. A simple, intuitive interface and onboard calculators streamline the process. Results appear within seconds and can be exported to a computer or downstream device. The Qubit 4 Fluorometer is designed for low-throughput processing, a few samples at a time. The Qubit Flex Fluorometer is designed for mid-throughput processing, quantifying up to eight samples at a time, with the flexibility to process other increments. Qubit Flex Fluorometers support add-on SAE software for 21 CFR Part 11 compliance support.

Description

Selection guide

Qubit 4 Fluorometer

Qubit Flex Fluorometer

Product summary
Highly accurate, easy-to-use quantification of a single sample of DNA, RNA, or protein Highly accurate, easy-to-use quantification of a up to eight samples of DNA, RNA, or protein

Detailed comparison table

Feature Qubit 4 Fluorometer Qubit Flex Fluorometer
Sample throughput 1 sample in 3 seconds 1–8 samples in 3 seconds
User interface 5.7-inch color touchscreen 8-inch color touchscreen
Onboard calculators Reagent calculator Reagent calculator
Assay Range calculator

Molarity calculator

Normalization calculator

System Check Qubit 4 System Verification Assay Kit Qubit Flex System Verification Assay Kit
Fluorometer mode Yes Yes
Programmable open format Yes, MyQubit No
Instrument footprint 13.6 x 25 x 5.5 cm

5.4 x 10 x 2.2 in.

18.6 x 28.2 x 10.3 cm

7.3 x 11.1 x 4.1 in.

Sample data storage 1,000 samples 10,000 samples
Data export
  • Wi-Fi to Thermo Fisher Connect or network drive
  • USB drive
  • Direct to computer via USB cable
  • Wi-Fi to Thermo Fisher Connect or network drive
  • USB drive
  • Direct to computer via Ethernet cable
Light sources Blue LED (peak ~470 nm)
Red LED (peak ~635 nm)
Blue LED (peak ~460–480 nm)
Red LED (peak ~620–640 nm)
Excitation filters Blue LED (430–495 nm)
Red LED (600–645 nm)
Blue LED (456–484 nm)
Red LED (612–644 nm)
Emission filters Green (510–580 nm)
Red (665–720 nm)
Green (513–563 nm)
Far-red (671–693 nm)
21 CFR Part 11 software support Not available Optional add-on SAE software
Additional information
Manufacturer