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Can We Replicate the Complexity of Human Bone Marrow in the Lab?

For years, the bone marrow’s intricate ecosystem—essential for blood formation and immune regulation—has remained elusive to researchers trying to study it outside the human body. Now, a groundbreaking protocol offers a solution by enabling the creation of 3D human bone marrow organoids from stem cells, setting the stage for transformative advances in disease modeling and drug discovery.
This innovative approach uses human induced pluripotent stem cells (hiPSCs), cultured in StemFlex™ Medium (A3349401) with RevitaCell™ Supplement (A2644501) supplementation and finally matured using StemPro™-34 SFM (10639011) with KnockOut™ Serum Replacement (10828028) with specific growth factors and cytokines, to generate vascularized, multi-lineage organoids in just 18 days. These bone marrow replicas combine hematopoietic, stromal, and endothelial cells in an architecture that closely mimics natural bone marrow, providing a more accurate model than ever before. Such organoids allow scientists to explore the dynamics of healthy and diseased bone marrow, offering new hope for studying conditions like myeloid and lymphoid cancers, which have been challenging to replicate in traditional culture systems.
Beyond understanding disease, these organoids create opportunities for drug testing—already showing potential for investigating treatments that target fibrosis and other bone marrow disorders. This protocol paves the way for creating personalized models that could dramatically speed up the discovery of tailored treatments, marking an exciting step forward in hematology research.

Olijnik, A.-A., Rodriguez-Romera, A., Wong, Z. C., Shen, Y., Reyat, J. S., Jooss, N. J., Rayes, J., Psaila, B., & Khan, A. O. (2024). Generating human bone marrow organoids for disease modeling and drug discovery. Nature Protocols, 19, 2117–2146. https://doi.org/10.1038/s41596-024-00971-7

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