Transfer methods
There are a variety of methods for transfer, including diffusion transfer, capillary transfer, heat-accelerated convectional transfer, vacuum blotting, and electroblotting (electrotransfer). Among these methods, electroblotting has emerged as the most popular and highly used for western blotting because it is faster and more efficient than the other methods. There are three ways to electrotransfer proteins from SDS-PAGE or native gels to membranes:
- Wet electroblotting (traditional wet or tank transfer)
- Semi-dry electroblotting (semi-dry transfer)
- Dry electroblotting (dry transfer)
Wet transfer | Semi-dry transfer | Dry transfer | ||
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![]() Mini Blot Module |
![]() XCell II Blot Module |
![]() SureLock Tandem Midi Blot Module |
![]() Power Blotter Systems |
![]() iBlot 3 Western Blot Transfer System |
Capacity: 1 mini gel per blot module; 1–2 blot modules per tank |
Capacity: up to 2 mini blots |
Capacity: 1 midi gel per blot module; 1–2 blot modules per tank |
Capacity: 1–4 mini or 1–2 midi gels |
Capacity: 1–4 mini or 1–2 midi gels |
Transfer time: 60 min | Transfer time: 60–120 min |
Transfer time: 30 min |
Transfer time: 5–10 min |
Transfer time: as few as 3 min |
Blotting area: 9 x 9 cm |
Blotting area: 9 x 9 cm |
Blotting area: 9.2 x 14.4 cm |
Blotting area: 10 x 18 cm or 21 x 22.5 cm |
Blotting area: 8.5 x 13.5 cm |
Blotting area: Transfer buffer volume: 220 mL per blot module |
Transfer buffer volume: 200 mL |
Transfer buffer volume: 300 mL per blot module |
Transfer buffer volume: Pre-cut membranes & filters: 50–100 mL; Pre-assembled Select transfer stacks: buffer not required |
Transfer buffer volume: Buffer not required |
Power supply: External | Power supply: External | Power supply: External |
Power supply: Internal | Power supply: Internal |
Required equipment: Mini Gel Tank: capacity for up to 2 Mini Blot Modules |
Required equipment: XCell SureLock Mini-Cell: capacity for up to 2 blot modules |
Required equipment: SureLock Tandem Midi Gel Tank: capacity for up to 2 blot modules |
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