iBlot 3 western blot transfer system
The next-generation dry protein transfer device with ready-to-use consumables that can deliver high performance and convenience. Perform reliable, high-quality transfers fast—in as few as three minutes. In addition, two independently controlled transfer stations allow you to share the device with your coworker, simultaneously running a total of four mini gels or two midi gels with two different programs. With the iBlot 3 system, you can achieve as good or better protein transfer efficiency compared to wet tank transfer and other rapid transfer methods. The preassembled single-use consumables and ultrafast transfer methods help eliminate variability—enabling consistent and repeatable results. In addition, the robust design has built-in cooling to reduce heat buildup and facilitate run-to-run consistency.
Transfer methods
There are a variety of methods for transfer, including diffusion transfer, capillary transfer, heat-accelerated convectional transfer, vacuum blotting, and electroblotting (electrotransfer). Among these methods, electroblotting has emerged as the most popular and highly used for western blotting because it is faster and more efficient than the other methods. There are three ways to electrotransfer proteins from SDS-PAGE or native gels to membranes:
- Wet electroblotting (traditional wet or tank transfer)
- Semi-dry electroblotting (semi-dry transfer)
- Dry electroblotting (dry transfer)
| Wet transfer | Semi-dry transfer | Dry transfer | ||
|---|---|---|---|---|
 Mini Blot Module |
 XCell II Blot Module |
 SureLock Tandem Midi Blot Module |
 Power Blotter Systems |
 iBlot 3 Western Blot Transfer System |
| Capacity: 1 mini gel per blot module; 1–2 blot modules per tank |
Capacity: up to 2 mini blots |
Capacity: 1 midi gel per blot module; 1–2 blot modules per tank |
Capacity: 1–4 mini or 1–2 midi gels |
Capacity: 1–4 mini or 1–2 midi gels |
| Transfer time: 60 min | Transfer time: 60–120 min |
Transfer time: 30 min |
Transfer time: 5–10 min |
Transfer time: as few as 3 min |
| Blotting area: 9 x 9 cm |
Blotting area: 9 x 9 cm |
Blotting area: 9.2 x 14.4 cm |
Blotting area: 10 x 18 cm or 21 x 22.5 cm |
Blotting area: 8.5 x 13.5 cm |
| Blotting area: Transfer buffer volume: 220 mL per blot module |
Transfer buffer volume: 200 mL |
Transfer buffer volume: 300 mL per blot module |
Transfer buffer volume: Pre-cut membranes & filters: 50–100 mL; Pre-assembled Select transfer stacks: buffer not required |
Transfer buffer volume: Buffer not required |
| Power supply: External | Power supply: External | Power supply: External |
Power supply: Internal | Power supply: Internal |
| Required equipment: Mini Gel Tank: capacity for up to 2 Mini Blot Modules |
Required equipment: XCell SureLock Mini-Cell: capacity for up to 2 blot modules |
Required equipment: SureLock Tandem Midi Gel Tank: capacity for up to 2 blot modules |
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| Learn more | Learn more | Learn more | Learn more | Learn more |
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