iBright Imaging Systems
The Invitrogen iBright Imaging Systems offer multiple powerful features to help make imaging and analyzing western blots and gels easy. The high-resolution 9.1 MP camera and suite of automated features helps you produce publication quality data fast. The touchscreen interface is carefully crafted to provide a smooth image capture experience. The system’s built in software and our standalone iBright Analysis Software are designed to streamline the image analysis process. Straightforward total lane protein and house-keeping protein normalization workflows allow for simpler image analysis from start to finish, no matter what your experience level. Maximize your investment with specialty applications and 21CFR part 11 compliance support.
Specifications
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| Core essential western blot and gel imaging-transition from darkroom and film-based detection with ease | Expanded application support with many of the same high-performance specifications as the iBright FL1500 imager | Maximum application support including up to 4-channel multiplex western blot imaging | |
| Core imaging applications | |||
| Colorimetric stained protein gels |  |
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| Fluorescent stained protein gels | |
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| Fluorescent stained nucleic acid gels | |
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| Colorimetric stained membranes | Limited* | |
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| Chemiluminescent western blots | |
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| Colorimetric western blots | |
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| Fluorescent western blots | |
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| Specialty plate-based imaging applications | |||
| Fluorescent colonies (ex. GFP expressing) | |
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| Visible colonies (ex. crystal violet stained) | |
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| Qualitative visible imaging applications** | |||
| Opaque objects (e.g., 2D strips, TLC plates, leaf sections) | |
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| Camera | |||
| Detector | Cooled 16-bit CCD, 65,535 shades of gray | ||
| Resolution | 9.1 megapixels | ||
| Lens | Fixed, 25 mm, f/0.95 | ||
| Field of view | 22.5 x 18.0 cm (W x D) (image up to 4 mini blots or gels) | ||
| Binning modes | 1×1, 2×2, 4×4 (high-resolution/sensitivity settings) | 1×1, 2×2, 3×3, 4×4, 5×5, 6×6, 8×8 (maximum flexibility for adjusting resolution and sensitivity based on needs) | |
| Zoom | 1–2x, digital (digital zoom reduces the effective resolution of the zoomed image) | 1–8x (1–2x mechanical + 1–4x digital) (Mechanical zoom maximizes sensitivity by moving the camera closer to the sample stage, reducing focal length) | |
| System interface | |||
| Touchscreen | 12.1 inch capacitive LCD display, 1,024 x 768 pixels | ||
| Storage and connectivity | |||
| USB | 2 USB (2.0) | ||
| Network | Ethernet port, Connect (cloud-based) connectivity, optional Wi-Fi (adapter sold separately) | ||
| Image file format | G2i (proprietary), TIFF, JPG, PNG | ||
| Hard drive | 64 GB SSD | 256 GB SSD | |
| System Hardware | |||
| Sample drawer and stage | Manually operated drawer with fixed stage | Automatic open/close drawer with automatic rotating stage (up to 10°) | |
| Filter set | 2 filters (0 excitation, 2 emission) | 4 filters (2 excitation, 2 emission) | 12 filter (6 excitation, 6 emission) |
| Illumination source |
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| System software | |||
| Automated features and algorithms |
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| Stand-alone analysis applications | iBright Analysis Software: desktop version (macOS™ or Windows™ operating systems) and Connect (cloud-based) version
iBright Analysis Software—Secure: (macOS™ or Windows™ operating systems) for 21 CFR part 11 compliance support |
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| Shipping, warranty, and upgrades | |||
| Dimensions | (L x W x H): 68 x 38 x 60 cm | ||
| Weight | Approximately 47 kg (105 lb) | Approximately 50 kg (110 lbs) | |
| Warranty | 1 year from date of purchase | 2 years from date of purchase | |
| Upgradability | No | Yes, to FL1500 imager | No. |
* Membranes must be imaged when wet, using Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes or Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes.
** Applications provide a qualitative visualization of the object or confirmation of the presence of signal. Not recommended for quantitation.
*** Smart Range HDR available in chemiluminescent blot mode only.
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