PowerFlex™ Thermal Cycler, 3×32-well

Ultimate Performance, Compatibility, and Productivity for Routine and High-Throughput Molecular Workflows.

The Applied Biosystems PowerFlex Thermal Cycler is the ultimate day-to-day workhorse for standard research, academic, and routine testing laboratories, while delivering the premium performance required for high-throughput biotech and pharmaceutical pipelines. Engineered for maximum versatility, the PowerFlex system features a unique 3 x 32-well independent block format that allows individual researchers, technicians, or students to run completely distinct thermal protocols simultaneously on a single compact instrument. This eliminates laboratory scheduling bottlenecks and optimizes bench space without compromising on scientific precision. With an exceptional block ramp rate of up to 7.0°C/sec, a stringent temperature accuracy of ±0.15°C, and a superior well-to-well uniformity of <0.4°C (at 95°C), the system ensures absolute consistency for routine applications. Additionally, its redesigned architecture offers seamless compatibility with 96-well fully skirted, semi-skirted, and non-skirted PCR plates, making it fully ready for integration into automated liquid handling and robotic screening pipelines.

Key Features & Benefits

• Accelerated Thermal Performance: Reaches an impressive block ramp rate of up to 7.0°C/sec and a maximum sample ramp rate of 6.2°C/sec, significantly driving down total protocol run times and accelerating daily sample throughput.

• True Multi-User Protocol Flexibility: Equipped with a versatile 3 x 32-well block format (0.2 mL) with fully independent control, allowing up to three different researchers to configure and run completely distinct thermal profiles at the same time.

• Broad Consumable Compatibility: Upgraded block design provides out-of-the-box compatibility with a wide range of PCR plate types, including fully skirted, semi-skirted, and non-skirted 96-well formats, as well as 8-tube strips and individual tubes.

• Seamless Simulation Mode: Allows running protocols under the exact same conditions as legacy thermal cyclers, ensuring a smooth and rapid transition of established protocols from the ProFlex PCR System without requiring re-optimization.

• VeriFlex Block Technology: Utilizes multiple independent Peltier elements (featuring 3 temperature zones per 32-well block) to enable precise linear temperature control for fast and accurate PCR optimization.

• Intuitive Interface and Enlarged Screen: Features an adjustable 10.1-inch color TFT touchscreen (20% larger than the ProFlex model) with an intuitive interface for effortless, interactive programming.

• Onboard Smart Help Diagnostics: Includes built-in automated troubleshooting capabilities for fast and accurate resolution of technical questions, ensuring maximal instrument uptime.

• Secure Remote Access: Supported by the Thermo Fisher Connect Platform, enabling secure remote monitoring and access anytime, anywhere from a mobile device or desktop to design protocols, check run status, and download experimental reports.

• Compact, Space-Saving Footprint: Optimized hardware design delivers a 20% smaller footprint compared to the previous ProFlex platform, freeing up valuable laboratory bench space.

Applications The PowerFlex Thermal Cycler is purpose-built to support core molecular biology workflows across multiple research and industrial application fields such as standard cloning, genotyping, cloning sequencing, and mutagenesis workflows and advanced assay development and screening optimization within biotechnology and pharmaceutical R&D facilities.