CTS™ Xenon™ Electroporation System

The Gibco™ CTS™ Xenon™ Electroporation System is a closed, modular, large-scale platform that offers full control over electroporation parameters for optimal performance. The system delivers exceptional transfection performance and viability, enabling electroporation of up to 2.5 billion cells in a 25 mL run to support both process development and manufacturing work in clinical cell therapy. Utilizing a user-programmable interface, it provides the flexibility needed to optimize protocols for a variety of cell types and payloads, ensuring high-quality results from research through commercialization.

High-Performance, Scalable Non-Viral Delivery for Clinical Manufacturing

The CTS Xenon System is specifically engineered to meet the rigorous demands of CAR T cell therapy and other advanced immunotherapies. By providing a closed and automated non-viral delivery workflow, it addresses critical challenges in cell therapy manufacturing, ensuring high-quality gene modification for both autologous and allogeneic cell-based products. The system allows for efficient delivery of various payloads, including DNA, RNA, and proteins, while providing the robustness needed for reliable therapeutic production.

Exceptional Transfection Performance and Viability

• High Efficiency: Achieve up to 90% gene knockout and up to 80% cell viability when used with Gibco CTS TrueCut Cas9 Protein.

• Proven Performance: Benchmarked against the Invitrogen Neon Transfection System, the CTS Xenon system enables seamless translation to large-scale processing without a drop in performance.

• Rapid Processing: Capable of transfecting up to 2.5 billion T cells in a 25 mL volume in less than 25 minutes.

• Precision Control: Delivers consistent results through an embedded graphical user interface (GUI) that allows for full optimization of electroporation protocols.

Integrated Fluidics and Parameter Management

• Automated Processing: Features intuitive software to manage all instrument actions, including voltage, pulse width, and pulse number.

• Full Control: Users can customize parameters such as voltage (500–2,500 V), pulse width (1–30 ms), and number of pulses (1–10) to suit specific cell types.

• Precooling Technology: Integrated Peltier technology for precooling (10–30°C) ensures optimal temperature control during the electroporation process.

Versatile Scalability and Optimized Consumables

• Scalable Consumables: Offers the CTS Xenon SingleShot chamber for rapid 1 mL process development and the CTS Xenon MultiShot cartridge for consistent, closed-system manufacturing volumes up to 25 mL.

• Flexible Buffers: Compatible with specialized buffers, including CTS Xenon Genome Editing Buffer and Lower Conductivity Buffer, designed specifically for high-energy electroporation needs.

• Seamless Translation: Facilitates a direct path from discovery to clinical manufacturing, allowing for process refinement at small scale before transitioning to large-scale production.

GMP-Compliant Manufacturing and Connectivity

• Sterile Environment: Closed, single-use MultiShot cartridges enable sterile processing within Class C cleanroom facilities.

• Regulatory Compliance: Manufactured in ISO 13485-certified facilities with software options designed to help enable 21 CFR Part 11 compliance.

• Digital Integration: Features an OPC UA interface for seamless communication with MES/LIMS platforms and DeltaV systems via CTS Cellmation Software.