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Optima AUC: Analytical Ultracentrifuge
AUC enables researchers two complementary views of solution behavior. Sedimentation velocity provides first-principle, hydrodynamic information about the size and shape of molecules. Sedimentation equilibrium provides the same type of information about the solution molar masses, stoichiometries, association constants and solution nonideality. Scientist can investigate nearly any type of molecule or particle over a wide range of concentrations and in a diverse variety of solvents and characterize samples in their native state under biologically relevant solution conditions.
Extracellular Vesicles
AUC can be an effective method to determine
EV composition and concentration.
Nanoparticle Analysis
AUC allows resolution of up to 1nm. Provides population statistics and determines the shape
of the particle.
Carbon Nanotubes
AUC can distinguish between more than 300 different species of carbon nanotubes, their length, purity and integrity.
Drug Discovery
Analytical ultracentrifugation allows characterization of:
•Conjugation efficiency of Antibody -Drug conjugates
•Toxicology lot evaluation
•API and drug formulation stability and lot release
•Formulation stress testing
Stoichiometry
AUC can distinguish between hydrodynamically unresolved materials. Elucidating the relative amounts of species in a co-sedimenting complex. In conjunction with additional hydrodynamic information- True Stoichiometry can be obtained.
Aggregation Analysis
AUC allows non disruptive study of protein interactions in almost any buffer.
•Determination of “s” and “D”. Size distributions are not affected
•Differentiates reversible and irreversible aggregation
•Ternary interactions. Simultaneous determination of: Kd, Koff, concentration,
size and stoichiometry
Structural Analysis of Proteins
AUC is useful in determining such parameters as Polydispersity and Aggregation.
Allows rigorous quantitative determination of hydrodynamic and thermodynamic properties.
Complementary to structural biology.
AAV
AUC allows:
•Determine viral assembly state or homogeneity (empty, full or oligomer), Empirically quantify aggregation
•Determine sub- particle contamination
•Mass quantification to accurately measure genetic payload
Sedimentation Analysis
AUC allows the determination of Size, Anisotropy and Density. Dynamics, Composition and Conformation.