Real-Time PCR Consumables & Reagents
A variety of reagents, controls, and kits (both Regulated and For Research Use Only) suitable for performing Real Time qPCR. This category includes products spanning a wide variety of applications, including but not limited to; gene expression analysis, genotyping, allelic discrimination, single nucleotide polymorphism (SNP) detection, microbe and pathogen detection. These products utilize TaqMan and SYBR chemistries for one-step or two-step amplification and quantitation of analytes via RT-qPCR.
Description
Which real-time chemistry is right for you?
| SYBR Green–based detection | TaqMan-based detection | ||
|---|---|---|---|
|
Uses Applied Biosystems™ SYBR™ Green dye (a dsDNA binding dye) to detect PCR product as it accumulates during PCR.
|
Uses a fluorogenic probe specific to target gene to detect target as it accumulates during PCR.
|
||
| Specificity | Medium* | High | |
| Sensitivity-low # of copies | Variable* | 1-10 copies | |
| Reproducibility | Medium* | High | |
| Multiplexing |  |
||
| Predesigned assays | |
||
| Typically requires user design, experimental optimization | |
||
| Gene expression | Low level of quantitation | High level of quantitation | |
| Applications | Gene expression DNA quantitation (pathogen detection) CHiP |
Gene expression DNA quantitation CHiP SNP genotyping Copy number variation Pathway analysis microRNA & small RNAs Mutation detection Protein analysis Multiplexing |
|
| SYBR Green primers SYBR Green master mixes |
TaqMan Assays TaqMan master mixes |
Tags: PCR, qPCR, SYBR, TAQMAN, gene expression, genotyping, microRNA, mutation detection, copy number
Additional information
| Manufacturer |
|---|
