Which real-time chemistry is right for you?
| SYBR Green–based detection | TaqMan-based detection | ||
|---|---|---|---|
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Uses Applied Biosystems™ SYBR™ Green dye (a dsDNA binding dye) to detect PCR product as it accumulates during PCR.
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Uses a fluorogenic probe specific to target gene to detect target as it accumulates during PCR.
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| Specificity | Medium* | High | |
| Sensitivity-low # of copies | Variable* | 1-10 copies | |
| Reproducibility | Medium* | High | |
| Multiplexing |  |
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| Predesigned assays | |
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| Typically requires user design, experimental optimization | |
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| Gene expression | Low level of quantitation | High level of quantitation | |
| Applications | Gene expression DNA quantitation (pathogen detection) CHiP |
Gene expression DNA quantitation CHiP SNP genotyping Copy number variation Pathway analysis microRNA & small RNAs Mutation detection Protein analysis Multiplexing |
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| SYBR Green primers SYBR Green master mixes |
TaqMan Assays TaqMan master mixes |
Tags: PCR, qPCR, SYBR, TAQMAN, gene expression, genotyping, microRNA, mutation detection, copy number
